Abstract：
Nanoliposomes are drug delivery systems that improve bioavailability by encapsulating therapeutic agents. The main objective of this study was to investigate the effects of nanoliposomal (NL) formulation on enhancing the bioavailability of essential oil. The essential oil of Satureja hortensis (SHO) was encapsulated in nanoliposomes (SHNLs). Physicochemical characterizations of NL formulations (size, charge, polydispersity index [PDI]) were evaluated by dynamic light scattering technique. The nanoliposome encapsulation efficiency (EE) was calculated as 89.90%. The prepared bionanosystems demonstrated significant antibacterial activities against Escherichia coli ATCC 10536, Pseudomonas aeruginosa ATCC 15442, and Staphylococcus aureus ATCC as determined by the agar diffusion method and microdilution tests. Minimum inhibitory concentration (MIC) values for SHNLs were found to be 5.187 µg/µL for E. coli and 2.59 µg/µL for both P. aeruginosa and S. aureus. Importantly, despite the lower substance content, both SHNLs and SHO exhibited comparable antibacterial activity against all tested strains. Furthermore, in order to determine the toxicity profile and possible effects on DNA damage or repair both the genotoxic and antigenotoxic effects of SHNLs were assessed using the cytokinesis-blocked micronucleus (CBMN) method in human lymphocyte cultures. The experimental data collectively indicate that the NL formulation of the S. hortensis essential oil enhances antibacterial activities and provides genoprotective effects against DNA damage. This highlights the significance of liposomal formulations of antioxidants in augmenting their biological activity. The results indicate that SHNLs can be a safe antibacterial agent for the pharmaceutical industry.
摘要：
纳米脂质体是通过包封治疗剂来提高生物利用度的药物递送系统。本研究的主要目的是研究纳米脂质体（NL）制剂对提高精油生物利用度的影响。Saturejahortensis（SHO）的精油被封装在纳米脂质体（SHNTs）中。NL制剂的物理化学表征(尺寸，charge,多分散指数[PDI]）通过动态光散射技术进行评估。纳米脂质体包封效率(EE)计算为89.90%。通过琼脂扩散法和微量稀释试验测定，制备的bionanosystems对大肠杆菌ATCC10536，铜绿假单胞菌ATCC15442和金黄色葡萄球菌ATCC具有显着的抗菌活性。发现SHNL的最小抑制浓度（MIC）值对于大肠杆菌为5.187μg/μL，对于铜绿假单胞菌和金黄色葡萄球菌为2.59μg/μL。重要的是,尽管物质含量较低，SHNTs和SHO对所有测试菌株均表现出相当的抗菌活性。此外，为了确定毒性谱和对DNA损伤或修复的可能影响,在人淋巴细胞培养物中使用胞质分裂阻断微核(CBMN)方法评估了SHNLs的遗传毒性和抗遗传毒性作用.实验数据共同表明,S.hotensis精油的NL制剂增强抗菌活性并提供针对DNA损伤的基因保护作用。这突出了抗氧化剂的脂质体制剂在增强其生物活性方面的重要性。结果表明,SHNLs可以是制药工业的安全抗菌剂。
