Abstract：
Notwithstanding the several investigations of the hydroxy fatty acids (hFAs)\' physiological functions, studies focusing on their anti-obesity effects are limited. This study investigated the anti-obesity effects of 4 hFAs-10-hydroxy stearic acid (10-hSA), 12-hydroxy stearic acid (12-hSA), 9,12-hydroxy stearic acid (9,12-dhSA), and 12-hydroxy oleic acid (12-hOA)-on the 3T3-L1 cells. All hFAs suppressed lipid accumulation, with 10-hSA and 12-hOA exhibiting the strongest suppression, followed by 12-hSA and 9,12-hSA. This trend was similar to that observed for the glycerol-3-phosphate dehydrogenase (GPDH) activity level. Contrastingly, only 9,12-dhSA suppressed cell viability. The mRNA levels of HK1 and Aldoa were markedly suppressed by 10-hSA and 12-hSA compared to the control. Additionally, mRNA expression of Gyk was considerably suppressed by 12-hSA. Thus, all hFAs suppressed lipid accumulation by suppressing GPDH activity, although their molecular mechanisms were different. These findings will aid the application of hFAs in the food and medical industries.
摘要：
尽管对羟基脂肪酸（hFA）的生理功能进行了多次研究，关注其抗肥胖作用的研究是有限的。这项研究调查了四种hFA的抗肥胖作用，10-羟基硬脂酸(10-hSA),12-羟基硬脂酸(12-hSA),9,12-羟基硬脂酸（9,12-dhSA），和12-羟基油酸（12-hOA），在3T3-L1细胞上。所有hFAs抑制脂质积累，10-hSA和12-hOA表现出最强的抑制作用，其次是12-hSA和9,12-hSA。这种趋势与观察到的甘油-3-磷酸脱氢酶(GPDH)活性程度相似。相反，只有9,12-dhSA抑制细胞活力。与对照组相比，10-hSA和12-hSA明显抑制了HK1和Aldoa的mRNA水平。此外,12-hSA显著抑制了Gyk的mRNA表达。因此，所有hFAs都通过抑制GPDH活性来抑制脂质积累，尽管它们的分子机制不同。这些发现将有助于hFA在食品和医疗行业的应用。
