Abstract:
Notwithstanding the several investigations of the hydroxy fatty acids (hFAs)\' physiological functions, studies focusing on their anti-obesity effects are limited. This study investigated the anti-obesity effects of 4 hFAs-10-hydroxy stearic acid (10-hSA), 12-hydroxy stearic acid (12-hSA), 9,12-hydroxy stearic acid (9,12-dhSA), and 12-hydroxy oleic acid (12-hOA)-on the 3T3-L1 cells. All hFAs suppressed lipid accumulation, with 10-hSA and 12-hOA exhibiting the strongest suppression, followed by 12-hSA and 9,12-hSA. This trend was similar to that observed for the glycerol-3-phosphate dehydrogenase (GPDH) activity level. Contrastingly, only 9,12-dhSA suppressed cell viability. The mRNA levels of HK1 and Aldoa were markedly suppressed by 10-hSA and 12-hSA compared to the control. Additionally, mRNA expression of Gyk was considerably suppressed by 12-hSA. Thus, all hFAs suppressed lipid accumulation by suppressing GPDH activity, although their molecular mechanisms were different. These findings will aid the application of hFAs in the food and medical industries.
摘要:
尽管对羟基脂肪酸(hFA)的生理功能进行了多次研究,关注其抗肥胖作用的研究是有限的。这项研究调查了四种hFA的抗肥胖作用,10-羟基硬脂酸(10-hSA),12-羟基硬脂酸(12-hSA),9,12-羟基硬脂酸(9,12-dhSA),和12-羟基油酸(12-hOA),在3T3-L1细胞上。所有hFAs抑制脂质积累,10-hSA和12-hOA表现出最强的抑制作用,其次是12-hSA和9,12-hSA。这种趋势与观察到的甘油-3-磷酸脱氢酶(GPDH)活性程度相似。相反,只有9,12-dhSA抑制细胞活力。与对照组相比,10-hSA和12-hSA明显抑制了HK1和Aldoa的mRNA水平。此外,12-hSA显著抑制了Gyk的mRNA表达。因此,所有hFAs都通过抑制GPDH活性来抑制脂质积累,尽管它们的分子机制不同。这些发现将有助于hFA在食品和医疗行业的应用。