METHODS: Needle biopsy samples were acquired using FNAB, CNB and USeFNAB on ex vivo human tonsils (N = 10). The tissue yield and quality were quantified by weight measurement and blinded pathologists\' assessments. The biopsy methods were then compared.
RESULTS: The results revealed sample mass increases of, on average, 2.3- and 5.4-fold with USeFNAB compared with the state-of-the-art FNAB and CNB, respectively. The quality of tissue fragments collected by USeFNAB was equivalent to that collected by the state-of-the-art methods in terms of morphology and immunohistochemical stainings made from cell blocks as judged by pathologists.
CONCLUSIONS: Our study indicates that USeFNAB is a promising method that could improve tissue yield to ensure sufficient material for ancillary histochemical and molecular studies for diagnostic pathology, thereby potentially increasing diagnostic accuracy.
方法:使用FNAB获取穿刺活检样本,离体人扁桃体上的CNB和USeFNAB(N=10)。通过体重测量和盲法病理学家评估来量化组织产量和质量。然后比较活检方法。
结果:结果显示,平均而言,与最先进的FNAB和CNB相比,UseFNAB为2.3倍和5.4倍,分别。从病理学家判断的细胞块的形态学和免疫组织化学染色方面,由UseFNAB收集的组织碎片的质量与通过最先进的方法收集的组织碎片的质量相同。
结论:我们的研究表明,UseFNAB是一种有前途的方法,可以提高组织产量,以确保有足够的材料用于辅助组织化学和分子研究的诊断病理学,从而潜在地提高诊断准确性。