Abstract：
OBJECTIVE: Needle biopsy is a common technique used to obtain cell and tissue samples for diagnostics. Currently, two biopsy methods are widely used: (i) fine-needle aspiration biopsy (FNAB) and (ii) core needle biopsy (CNB). However, these methods have limitations. Recently, we developed ultrasound-enhanced fine-needle aspiration biopsy (USeFNAB), which employs a needle that flexurally oscillates at an ultrasonic frequency of ∼32 kHz. The needle motion contributes to increased tissue collection while preserving cells and tissue constructs for pathological assessment. Previously, USeFNAB has been investigated only in ex vivo animal tissue. The present study was aimed at determining the feasibility of using USeFNAB in human epithelial and lymphoid tissue.
METHODS: Needle biopsy samples were acquired using FNAB, CNB and USeFNAB on ex vivo human tonsils (N = 10). The tissue yield and quality were quantified by weight measurement and blinded pathologists\' assessments. The biopsy methods were then compared.
RESULTS: The results revealed sample mass increases of, on average, 2.3- and 5.4-fold with USeFNAB compared with the state-of-the-art FNAB and CNB, respectively. The quality of tissue fragments collected by USeFNAB was equivalent to that collected by the state-of-the-art methods in terms of morphology and immunohistochemical stainings made from cell blocks as judged by pathologists.
CONCLUSIONS: Our study indicates that USeFNAB is a promising method that could improve tissue yield to ensure sufficient material for ancillary histochemical and molecular studies for diagnostic pathology, thereby potentially increasing diagnostic accuracy.
METHODS: Needle biopsy samples were acquired using FNAB, CNB and USeFNAB on ex vivo human tonsils (N = 10). The tissue yield and quality were quantified by weight measurement and blinded pathologists\' assessments. The biopsy methods were then compared.
RESULTS: The results revealed sample mass increases of, on average, 2.3- and 5.4-fold with USeFNAB compared with the state-of-the-art FNAB and CNB, respectively. The quality of tissue fragments collected by USeFNAB was equivalent to that collected by the state-of-the-art methods in terms of morphology and immunohistochemical stainings made from cell blocks as judged by pathologists.
CONCLUSIONS: Our study indicates that USeFNAB is a promising method that could improve tissue yield to ensure sufficient material for ancillary histochemical and molecular studies for diagnostic pathology, thereby potentially increasing diagnostic accuracy.
摘要：
目的：穿刺活检是一种用于获取细胞和组织样本以进行诊断的常用技术。目前,两种活检方法被广泛使用：（i）细针穿刺活检（FNAB）和（ii）芯针穿刺活检（CNB）。然而,这些方法有局限性。最近,我们开发了超声增强细针穿刺活检（USeFNAB），它采用了一种在~32kHz的超声波频率下弯曲振荡的针。针运动有助于增加组织收集,同时保留细胞和组织构建体以用于病理评估。以前,仅在离体动物组织中研究了UseFNAB。本研究旨在确定在人类上皮和淋巴组织中使用UseFNAB的可行性。
方法：使用FNAB获取穿刺活检样本，离体人扁桃体上的CNB和USeFNAB(N=10)。通过体重测量和盲法病理学家评估来量化组织产量和质量。然后比较活检方法。
结果：结果显示，平均而言,与最先进的FNAB和CNB相比，UseFNAB为2.3倍和5.4倍，分别。从病理学家判断的细胞块的形态学和免疫组织化学染色方面，由UseFNAB收集的组织碎片的质量与通过最先进的方法收集的组织碎片的质量相同。
结论：我们的研究表明，UseFNAB是一种有前途的方法，可以提高组织产量，以确保有足够的材料用于辅助组织化学和分子研究的诊断病理学，从而潜在地提高诊断准确性。
方法：使用FNAB获取穿刺活检样本，离体人扁桃体上的CNB和USeFNAB(N=10)。通过体重测量和盲法病理学家评估来量化组织产量和质量。然后比较活检方法。
结果：结果显示，平均而言,与最先进的FNAB和CNB相比，UseFNAB为2.3倍和5.4倍，分别。从病理学家判断的细胞块的形态学和免疫组织化学染色方面，由UseFNAB收集的组织碎片的质量与通过最先进的方法收集的组织碎片的质量相同。
结论：我们的研究表明，UseFNAB是一种有前途的方法，可以提高组织产量，以确保有足够的材料用于辅助组织化学和分子研究的诊断病理学，从而潜在地提高诊断准确性。
