Abstract：
BACKGROUND: The abuse of the Phencyclidine-type substances, especially ketamine is a serious problem worldwide, and retrospective analysis are important for both the analysis and the identification of forms of drug abuse. The current major analytical methods, while all excellent in terms of accuracy, are time- and reagent-consuming. This depletion is made even more unfortunate by the fact that a large number of samples are negative in retrospective analyses. It is clear that a set of methods that can be analyzed both accurately and quickly need to be developed and applied to the screening and analysis of large quantities of samples.
RESULTS: We described a urine test based on acoustic ejection mass spectrometry, which allows precise injection at very low volumes and near 1 ejection s-1 and data acquisition. The confidence in identification was increased by the characterization of the abundance ratio of the two pairs of ions. Urine samples could be diluted with water and loaded into a 384-well plate for sampling without complicated sample preparation. The sample in the transparent 384-well plate was pre-scanned by the laser, and then 20 nL droplets were ejected into the ion source for targeted analysis of 2 ion transitions per droplet totaling 9 targeted analytes in the sequence of acquisition methods. It took 90 min to screen 250 samples in this approach, yielding 10 ng mL-1 detection limits. Positive samples were further analyzed by UHPLC-MS/MS for confirmation and quantification of up to 36 analytes.
CONCLUSIONS: This was the first fast screening method for phencyclidine-type substances based on acoustic ejection mass spectrometry, which greatly reduces the analytical time, and can accomplish in 1.5 h what UHPLC-MS/MS needs 3 days to complete. And the samples can be analyzed without complicated sample preparation, and also can obtain good detectability. It was applied to a short-term retrospective analysis in Shanghai, and its accuracy was also extremely high.
RESULTS: We described a urine test based on acoustic ejection mass spectrometry, which allows precise injection at very low volumes and near 1 ejection s-1 and data acquisition. The confidence in identification was increased by the characterization of the abundance ratio of the two pairs of ions. Urine samples could be diluted with water and loaded into a 384-well plate for sampling without complicated sample preparation. The sample in the transparent 384-well plate was pre-scanned by the laser, and then 20 nL droplets were ejected into the ion source for targeted analysis of 2 ion transitions per droplet totaling 9 targeted analytes in the sequence of acquisition methods. It took 90 min to screen 250 samples in this approach, yielding 10 ng mL-1 detection limits. Positive samples were further analyzed by UHPLC-MS/MS for confirmation and quantification of up to 36 analytes.
CONCLUSIONS: This was the first fast screening method for phencyclidine-type substances based on acoustic ejection mass spectrometry, which greatly reduces the analytical time, and can accomplish in 1.5 h what UHPLC-MS/MS needs 3 days to complete. And the samples can be analyzed without complicated sample preparation, and also can obtain good detectability. It was applied to a short-term retrospective analysis in Shanghai, and its accuracy was also extremely high.
摘要：
背景：苯环利定类物质的滥用，特别是氯胺酮是世界范围内的一个严重问题，和回顾性分析对于分析和识别药物滥用形式都很重要。目前主要的分析方法，虽然在准确性方面都很好，是耗时和耗时的试剂。由于大量样品在回顾性分析中呈阴性，这一事实使这种消耗更加不幸。显然，需要开发一套既可以准确又可以快速分析的方法，并将其应用于大量样品的筛选和分析。
结果：我们描述了一种基于声喷射质谱的尿液测试，这允许在非常低的体积和接近1喷射s-1的精确注射和数据采集。通过表征两对离子的丰度比，提高了鉴定的置信度。尿液样品可以用水稀释并装入384孔板中进行采样，而无需复杂的样品制备。用激光对透明384孔板中的样品进行预扫描，然后将20nL液滴喷射到离子源中,用于以采集方法的顺序对每个液滴2个离子跃迁进行靶向分析,总计9个靶向分析物。在这种方法中，筛选250个样品花了90分钟，产生10ngmL-1的检测限。通过UHPLC-MS/MS进一步分析阳性样品以确认和定量多达36种分析物。
结论：这是第一个基于声喷射质谱的苯环利定类物质的快速筛选方法，这大大缩短了分析时间，并且可以在1.5小时内完成UHPLC-MS/MS需要3天才能完成。并且可以分析样品，而无需复杂的样品制备，并且还可以获得良好的可检测性。它被应用于上海的短期回顾性分析，它的准确性也非常高。
结果：我们描述了一种基于声喷射质谱的尿液测试，这允许在非常低的体积和接近1喷射s-1的精确注射和数据采集。通过表征两对离子的丰度比，提高了鉴定的置信度。尿液样品可以用水稀释并装入384孔板中进行采样，而无需复杂的样品制备。用激光对透明384孔板中的样品进行预扫描，然后将20nL液滴喷射到离子源中,用于以采集方法的顺序对每个液滴2个离子跃迁进行靶向分析,总计9个靶向分析物。在这种方法中，筛选250个样品花了90分钟，产生10ngmL-1的检测限。通过UHPLC-MS/MS进一步分析阳性样品以确认和定量多达36种分析物。
结论：这是第一个基于声喷射质谱的苯环利定类物质的快速筛选方法，这大大缩短了分析时间，并且可以在1.5小时内完成UHPLC-MS/MS需要3天才能完成。并且可以分析样品，而无需复杂的样品制备，并且还可以获得良好的可检测性。它被应用于上海的短期回顾性分析，它的准确性也非常高。
