Abstract：
Aflatoxin B1 (AFB1) is known to inhibit growth, and inflict hepatic damage by interfering with protein synthesis. Allicin, has been acknowledged as an efficacious antioxidant capable of shielding the liver from oxidative harm. This study aimed to examine the damage caused by AFB1 on bovine hepatic cells and the protective role of allicin against AFB1-induced cytotoxicity. In this study, cells were pretreated with allicin before the addition of AFB1 for co-cultivation. Our findings indicate that AFB1 compromises cellular integrity, suppresses the expression of nuclear factor erythroid 2-related factor 2 (Nrf2). In addition, allicin attenuates oxidative damage to bovine hepatic cells caused by AFB1 by promoting the expression of the Nrf2 pathway and reducing cell apoptosis. In conclusion, the results of this study will help advance clinical research and applications, providing new options and directions for the prevention and treatment of liver diseases.
摘要：
已知黄曲霉毒素B1（AFB1）抑制生长，并通过干扰蛋白质合成造成肝损伤。大蒜素,已被认为是一种有效的抗氧化剂，能够保护肝脏免受氧化伤害。本研究旨在研究AFB1对牛肝细胞的损伤以及大蒜素对AFB1诱导的细胞毒性的保护作用。在这项研究中,在添加AFB1进行共培养之前，用大蒜素预处理细胞。我们的发现表明AFB1损害了细胞完整性，抑制核因子红系2相关因子2（Nrf2）的表达。此外,大蒜素通过促进Nrf2通路的表达和减少细胞凋亡来减轻AFB1对牛肝细胞的氧化损伤。总之,这项研究的结果将有助于推进临床研究和应用，为肝脏疾病的预防和治疗提供了新的选择和方向。
