OBJECTIVE: To gain deeper insights into the inflammatory response in the process of PCa.
METHODS: A total of 233 cases were collected, including 80 cases of prostate hyperplasia as disease control, 65 cases of postoperative prostate cancer and 36 cases of prostate cancer as PCa group. Additionally, 52 patients undergoing physical examinations during the same period were collected as the healthy control. The levels of 12 inflammatory cytokines in peripheral blood samples were analyzed using flow cytometric bead array technology. The levels of total prostate-specific antigen (TPSA) and free prostate-specific antigen (FPSA) in peripheral blood samples were analyzed using electrochemiluminescence technology.
RESULTS: Our findings revealed significant increases in serum IL-8 levels in PCa group compared to the healthy control group. Additionally, IL-6, IL-10, IFN-γ and IL-12p70 levels were markedly elevated in the PCa group compared to the disease control group (all p < 0.05). Conversely, the level of IL-4, TNF-α, IL-1β, IL-17A and IFN-α were lower in the PCa group compared to those in control group. Following surgery, the concentration of IL-6 decreased; whereas, the concentrations of IL-4, TNF-α, IL-17A, IL-1β, IL-12p70, and IFN-α increased, demonstrating significant differences (p < 0.05). The differential upregulation of IL-6 or downregulation of IL-17A in peripheral blood exhibited diagnostic efficacy in PCa patients. Moreover, we observed a significant increase in IL-17A levels, accompanied by decreased of IL-2, IL-4, IL-10, TNF-a, IFN-γ, IL-1β, and IL-12P70 in patients with distant metastasis.
CONCLUSIONS: The peripheral blood cytokines are closely associated with the occurrence and development of prostate cancer, especially the serum levels of IL-6 and IL-17A may be useful as potential predictors of PCa diagnosis.
目的:为了更深入地了解PCa过程中的炎症反应。
方法:共收集233例,包括80例前列腺增生作为疾病对照,术后前列腺癌65例,前列腺癌36例作为PCa组。此外,收集同期进行体检的52例患者作为健康对照。使用流式细胞术珠子阵列技术分析外周血样品中12种炎性细胞因子的水平。使用电化学发光技术分析外周血样品中总前列腺特异性抗原(TPSA)和游离前列腺特异性抗原(FPSA)的水平。
结果:我们的发现表明,与健康对照组相比,PCa组的血清IL-8水平显着增加。此外,与疾病对照组相比,PCa组的IL-6,IL-10,IFN-γ和IL-12p70水平显着升高(均p<0.05)。相反,IL-4,TNF-α,IL-1β,与对照组相比,PCa组的IL-17A和IFN-α较低。手术后,IL-6的浓度降低;然而,IL-4、TNF-α、IL-17A,IL-1β,IL-12p70和IFN-α增加,差异显著(p<0.05)。外周血中IL-6的差异上调或IL-17A的下调在PCa患者中具有诊断功效。此外,我们观察到IL-17A水平显着增加,伴随IL-2,IL-4,IL-10,TNF-α,IFN-γ,IL-1β,和IL-12P70在远处转移患者中的表达。
结论:外周血细胞因子与前列腺癌的发生发展密切相关。尤其是IL-6和IL-17A的血清水平可能是PCa诊断的潜在预测因子.