PDF(Pubmed)
Abstract：
Soil microbial communities perform critical ecosystem services through the collective metabolic activities of numerous individual organisms. Most microbes use corrinoids, a structurally diverse family of cofactors related to vitamin B12. Corrinoid structure influences the growth of individual microbes, yet how these growth responses scale to the community level remains unknown. Analysis of metagenome-assembled genomes suggests that corrinoids are supplied to the community by members of the archaeal and bacterial phyla Thermoproteota, Actinobacteria, and Proteobacteria. Corrinoids were found largely adhered to the soil matrix in a grassland soil, at levels exceeding those required by cultured bacteria. Enrichment cultures and soil microcosms seeded with different corrinoids showed distinct shifts in bacterial community composition, supporting the hypothesis that corrinoid structure can shape communities. Environmental context influenced both community- and taxon-specific responses to specific corrinoids. These results implicate corrinoids as key determinants of soil microbiome structure and suggest that environmental micronutrient reservoirs promote community stability.
摘要：
土壤微生物群落通过许多个体生物的集体代谢活动来执行关键的生态系统服务。大多数微生物使用Corrinoid，与维生素B12相关的辅助因子的结构多样化家族。Corrinoid结构影响个体微生物的生长，然而，这些增长反应如何达到社区水平仍然未知。宏基因组组装的基因组的分析表明，古细菌和细菌门Thermoproteota的成员向社区提供了类corrinoid，放线菌,和变形杆菌。在草地土壤中，发现Corrinoids主要粘附在土壤基质上，超过培养细菌所需的水平。用不同的corrinoid播种的富集培养物和土壤微观世界显示出细菌群落组成的明显变化，支持corrinoid结构可以塑造群落的假设。环境环境影响了社区和分类单元对特定corrinoid的反应。这些结果表明corrinoid是土壤微生物组结构的关键决定因素，并表明环境微量营养素库可促进群落稳定。
