PDF(Pubmed)
Abstract：
Hemorrhagic bowel syndrome (HBS) is characterized by a dissecting intramucosal hematoma at the small bowel, causing obstruction and severe hemorrhage in dairy cattle. Recent investigation revealed the presence of early-stage lesions in cows affected by HBS. These are presumed to be the initial stage of the hematoma, as both share unique dissection of the lamina muscularis mucosae (LMM) as histological hallmark. Early-stage lesions of HBS have not been characterized in greater detail, and neither has the hypothesis of mucosal abrasion as etiology been explored. Therefore, the first objective of the present study was to characterize the morphology of early-stage lesions, by gross examination, histochemistry, immunohistochemistry and transmission electron microscopy. The second objective was to determine the effect of mucosal abrasion to the small intestine in an ex vivo model. A total of 86 early-stage lesions from 10 cows with HBS were characterized. No underlying alterations at the LMM were evident which could explain their occurrence. However, degeneration at the ultrastructural level of the LMM smooth muscle cells was present in 3 of 4 lesions, it is however unclear whether this is primary or secondary. Bacteriological examination did not reveal any association with a specific bacterium. Experimental-induced and early-stage lesions were gross and histologically evaluated and scored in three cows with HBS and seven controls. Experimentally induced lesions in both affected cows and controls, were histologically very similar to the naturally occurring early-stage lesions. Altogether, the results are suggestive for mucosal trauma to play a role in the pathogenesis of HBS.
摘要：
肠出血性综合征（HBS）的特征是小肠粘膜内血肿，导致奶牛阻塞和严重出血。最近的调查显示，受HBS影响的奶牛存在早期病变。这些被认为是血肿的初始阶段，因为两者都共享粘膜肌层（LMM）的独特解剖作为组织学标志。尚未对HBS的早期病变进行更详细的表征。也没有探讨粘膜磨损作为病因的假设。因此,本研究的第一个目标是表征早期病变的形态，通过粗略检查，组织化学,免疫组织化学和透射电子显微镜。第二个目的是确定离体模型中粘膜磨损对小肠的影响。对来自10头HBS母牛的总共86个早期病变进行了表征。LMM没有明显的潜在改变，这可以解释它们的发生。然而,4个病灶中有3个存在LMM平滑肌细胞超微结构水平的变性，然而，目前还不清楚这是主要还是次要。细菌学检查未发现与特定细菌有任何关联。在三头HBS母牛和七个对照中对实验诱导和早期病变进行了总体和组织学评估和评分。在受影响的奶牛和对照中实验诱导的病变，组织学上与自然发生的早期病变非常相似。总之,该结果提示粘膜损伤在HBS的发病机制中起作用.
