PDF(Pubmed)
Abstract：
Humans benefit from a vast community of microorganisms in their gastrointestinal tract, known as the gut microbiota, numbering in the tens of trillions. An imbalance in the gut microbiota known as dysbiosis, can lead to changes in the metabolite profile, elevating the levels of toxins like Bacteroides fragilis toxin (BFT), colibactin, and cytolethal distending toxin. These toxins are implicated in the process of oncogenesis. However, a significant portion of the Bacteroides fragilis genome consists of functionally uncharacterized and hypothetical proteins. This study delves into the functional characterization of hypothetical proteins (HPs) encoded by the Bacteroides fragilis genome, employing a systematic in silico approach. A total of 379 HPs were subjected to a BlastP homology search against the NCBI non-redundant protein sequence database, resulting in 162 HPs devoid of identity to known proteins. CDD-Blast identified 106 HPs with functional domains, which were then annotated using Pfam, InterPro, SUPERFAMILY, SCANPROSITE, SMART, and CATH. Physicochemical properties, such as molecular weight, isoelectric point, and stability indices, were assessed for 60 HPs whose functional domains were identified by at least three of the aforementioned bioinformatic tools. Subsequently, subcellular localization analysis was examined and the gene ontology analysis revealed diverse biological processes, cellular components, and molecular functions. Remarkably, E1WPR3 was identified as a virulent and essential gene among the HPs. This study presents a comprehensive exploration of B. fragilis HPs, shedding light on their potential roles and contributing to a deeper understanding of this organism\'s functional landscape.
摘要：
人类受益于胃肠道中庞大的微生物群落，被称为肠道微生物群，数万亿。肠道微生物群的不平衡被称为生态失调，会导致代谢物分布的变化，提高毒素的水平，如脆弱拟杆菌毒素（BFT），Colibactin,和细胞致死膨胀毒素。这些毒素与肿瘤发生过程有关。然而,脆弱拟杆菌基因组的重要部分由功能上未表征和假设的蛋白质组成。这项研究深入研究了由脆弱拟杆菌基因组编码的假设蛋白质（HP）的功能特征，采用系统的计算机方法。针对NCBI非冗余蛋白质序列数据库，对总共379个HP进行了BlastP同源性搜索，导致162个与已知蛋白质缺乏同一性的HP。CDD-Blast鉴定了106个具有功能域的HPs，然后用Pfam注释，InterPro,超级家庭,SCANPROSITE,聪明,还有CATH.物理化学性质,如分子量，等电点,和稳定性指数，评估了60个HP，其功能结构域通过至少三个上述生物信息学工具鉴定。随后，亚细胞定位分析进行了检查，基因本体论分析揭示了不同的生物过程，细胞成分，和分子功能。值得注意的是,E1WPR3被鉴定为HP中的毒性和必需基因。本研究对脆弱芽孢杆菌HP进行了全面的探索，阐明它们的潜在作用，并有助于更深入地了解这种生物的功能景观。
