PDF(Pubmed)
Abstract：
Infection caused by the Humicola sp is extremely rare. We report the first case of fungal keratitis caused by Humicola pulvericola (H. pulvericola) in a 63-year-old man with a history of exposed to hot oil two weeks ago who developed keratitis. Direct examination of confocal microscopy and corneal scrapings showed fungal hyphae and isolates were identified by morphology and by sequencing the internal transcribed spacer region of ribosomal DNA. The in vitro antifungal susceptibilities of the case strain were tested for five antifungal agents. The results showed that the infectious agent was resistant towards fluconazole, caspofungin and amphotericin B; itraconazole and voriconazole were highly effective against H. pulvericola. He was diagnosed with H. pulvericola keratitis and treated with oral itraconazole and natamycin eyedrops. After one month of treatment, the lesion gradually improved, with the best-corrected visual acuity improving to 0.8.
摘要：
由Humicolasp引起的感染极为罕见。我们报告了第一例由腐殖质引起的真菌性角膜炎（H.fludicola）在一名63岁的男子中，该男子两周前曾接触过热油，患有角膜炎。共聚焦显微镜和角膜刮片的直接检查显示，通过形态学和核糖体DNA的内部转录间隔区测序鉴定了真菌菌丝和分离株。对五种抗真菌剂进行了病例菌株的体外抗真菌敏感性测试。结果表明，感染剂对氟康唑耐药，卡泊芬净和两性霉素B；伊曲康唑和伏立康唑对H.pudicola非常有效。他被诊断出患有H.praidicola角膜炎，并口服伊曲康唑和那他霉素滴眼液。经过一个月的治疗，病变逐渐好转,最佳矫正视力提高到0.8。
