UNASSIGNED: ICGA was performed on 20 male BALB/c mice under five wavelengths (900/1,000/1,100, /1,250/1,450 nm) to assess vascular resolution after ICG perfusion. A \"mirrored-L\" flap model with three angiosomes was established on another 20 male BALB/c mice, randomly divided into two equal groups. In Group A, a midline between angiosomes II and III was used as a border. In Group B, the points of the minimized choke vessel caliber marked according to the ICG signal at 1,450 nm wavelength (ICG1450) were connected. Necrotic area calculations, pathohistological testing, and statistical analysis were performed.
UNASSIGNED: The vascular structure was clearly observed at 1,450 nm wavelength, while the 900 to 1,100 nm failed to depict vessel morphology. Necrosis was beyond the borderline in 60% of Group A. Conversely, 100% of Group B had necrosis distal to the borderline. The number of choke vessels between angiosomes II and III was positively correlated with the necrotic area (%). The pathohistological findings supported the gross observation and analysis.
UNASSIGNED: ICG1450 can delineate the vessel structure in vivo and predict the viability of pedicled skin flaps using the choke vessel as the border between angiosomes.
■在五个波长(900/1,000/1,100,/1,250/1,450nm)下对20只雄性BALB/c小鼠进行ICGA,以评估ICG灌注后的血管分辨率。在另外20只雄性BALB/c小鼠上建立了具有三个血管小体的“镜像L”皮瓣模型,随机分为两组。A组,血管体II和III之间的中线用作边界。B组,连接根据ICG信号在1,450nm波长(ICG1450)标记的最小化扼流圈管径的点。坏死面积计算,病理组织学测试,并进行统计学分析。
■在1,450nm波长处清楚地观察到血管结构,而900至1,100nm未能描绘血管形态。60%的A组坏死超出了界限。相反,B组的100%在边界线远端有坏死。血管体II和III之间的窒息血管数量与坏死面积(%)呈正相关。病理组织学发现支持总体观察和分析。
■ICG1450可以在体内描绘血管结构,并使用扼流圈作为血管小体之间的边界来预测带蒂皮瓣的生存力。