PDF(Pubmed)
Abstract：
Disturbances in the oral microbiota may be due to several mechanisms and factors, such as smoking. An imbalance in oral bacteria may result in changes to the innate immune system and the development of periodontal disease. This study aimed to investigate the distribution of oral microbiota in smokers and non-smokers in a South African population using subgingival plaque samples. From the 128 recruited participants, 57 were identified as smokers (serum cotinine: >15 ng/ml). Analysis of 16S rRNA gene sequencing demonstrated significant differences between the two groups with a reduced abundance of Actinobacteria in smokers. Fusobacterium and Campylobacter were found in higher abundance, while a lower abundance of Leptotrichia, Actinomyces, Corynebacterium, and Lautropia were observed. This study highlighted significant differences in the oral microbiota of smokers, indicating an abundance of anaerobic gram-negative bacteria. These findings suggest that smoking allows certain oral microorganisms to gain dominance, thereby predisposing individuals to periodontal disease development and progression.
摘要：
口腔微生物群的紊乱可能是由于几种机制和因素。比如吸烟。口腔细菌的失衡可能导致先天免疫系统的变化和牙周病的发展。这项研究旨在使用牙龈下牙菌斑样本调查南非人群中吸烟者和非吸烟者口腔微生物群的分布。从128名招募的参与者中，57人被鉴定为吸烟者(血清可替宁:>15ng/ml)。16SrRNA基因测序的分析表明，两组之间存在显着差异，吸烟者中放线菌的丰度降低。梭菌和弯曲杆菌的丰度较高，而Leptotrichia的丰度较低，放线菌,棒状杆菌,观察到Lautropia。这项研究强调了吸烟者口腔微生物群的显着差异，表明有大量的厌氧革兰氏阴性细菌。这些发现表明，吸烟可以使某些口腔微生物获得优势，从而诱发个体牙周病的发展和进展。
