Abstract：
Dosage compensation complex (DCC), which consists of five proteins and two non-coding RNAs roX, specifically binds to the X chromosome in males, providing a higher level of gene expression necessary to compensate for the monosomy of the sex chromosome in male Drosophila compared to the two X chromosomes in females. The MSL2 protein contains the N-terminal RING domain, which acts as an E3 ligase in ubiquitination of proteins and is the only subunit of the complex expressed only in males. Functional role of the two C-terminal domains of the MSL2 protein, enriched with proline (P-domain) and basic amino acids (B-domain), was investigated. As a result, it was shown that the B-domain destabilizes the MSL2 protein, which is associated with the presence of two lysines ubiquitination of which is under control of the RING domain of MSL2. The unstructured proline-rich domain stimulates transcription of the roX2 gene, which is necessary for effective formation of the dosage compensation complex.
摘要：
剂量补偿复合体（DCC），由五种蛋白质和两种非编码RNA组成，与男性的X染色体特异性结合，与雌性的两个X染色体相比，提供了更高水平的基因表达，以补偿雄性果蝇的性染色体的一元性。MSL2蛋白含有N端RING结构域,它在蛋白质的泛素化中充当E3连接酶，并且是仅在男性中表达的复合物的唯一亚基。MSL2蛋白的两个C端结构域的功能作用，富含脯氨酸（P域）和碱性氨基酸（B域），被调查。因此,研究表明，B结构域使MSL2蛋白不稳定，其与两个赖氨酸的存在相关,所述赖氨酸的泛素化处于MSL2的RING结构域的控制之下。非结构化的富含脯氨酸的结构域刺激roX2基因的转录，这是有效形成剂量补偿复合物所必需的。
