PDF(Pubmed)
Abstract：
BACKGROUND: Carious/Non-carious cervical lesions with gingival recessions may require both dental and periodontal reconstructive therapy, where flaps/grafts may be placed in contact with a dental filling material. Human Gingival Fibroblasts (HGF-1) response during the early phase of healing could vary according to the procedures employed to cure the dental composite. Moreover, oxygen diffusion into dental composite inhibits the polymerization reaction, creating an oxygen-inhibited layer (OIL) that presents residual unreacted monomers. The aim of this study was to assess the effect of different polishing techniques and OIL on HGF-1.
METHODS: Composite discs polished with different techniques (diamond rubber, abrasive discs and tungsten carbide burr) were used. An additional not polished smooth group obtained with and without OIL was used as control. Samples were physically characterized through the analysis of their hydrophilicity and surface topography through contact angle measurement and SEM, respectively; afterwards the biologic response of HGF-1 when cultured on the different substrates was analyzed in terms of cytotoxicity and gene expression.
RESULTS: The finishing systems caused alterations to the wettability, even if without a proportional relation towards the results of the proliferation essay, from which emerges a greater proliferation on surfaces polished with one-step diamond rubber and with abrasive discs as well as a direct effect of the glycerin layer, confirming that surface roughness can heavily influence the biological response of HGF-1.
CONCLUSIONS: Surfaces wettability as well as cellular behavior seem to be affected by the selection of the finishing system used to lastly shape the restoration. Especially, the presence of OIL act as a negative factor in the regards of human gingival fibroblasts. The present study may provide the first clinical instruction regarding the best polishing system of composite material when the restoration is placed directly in contact with soft tissue cells. Understanding HGF-1 behavior can help identifying the polishing treatment for direct restoration of carious/non-carious cervical lesions associated with gingival recessions.
METHODS: Composite discs polished with different techniques (diamond rubber, abrasive discs and tungsten carbide burr) were used. An additional not polished smooth group obtained with and without OIL was used as control. Samples were physically characterized through the analysis of their hydrophilicity and surface topography through contact angle measurement and SEM, respectively; afterwards the biologic response of HGF-1 when cultured on the different substrates was analyzed in terms of cytotoxicity and gene expression.
RESULTS: The finishing systems caused alterations to the wettability, even if without a proportional relation towards the results of the proliferation essay, from which emerges a greater proliferation on surfaces polished with one-step diamond rubber and with abrasive discs as well as a direct effect of the glycerin layer, confirming that surface roughness can heavily influence the biological response of HGF-1.
CONCLUSIONS: Surfaces wettability as well as cellular behavior seem to be affected by the selection of the finishing system used to lastly shape the restoration. Especially, the presence of OIL act as a negative factor in the regards of human gingival fibroblasts. The present study may provide the first clinical instruction regarding the best polishing system of composite material when the restoration is placed directly in contact with soft tissue cells. Understanding HGF-1 behavior can help identifying the polishing treatment for direct restoration of carious/non-carious cervical lesions associated with gingival recessions.
摘要：
背景：伴有牙龈凹陷的慢性/非龋齿宫颈病变可能需要牙科和牙周重建治疗，其中翼片/移植物可以放置成与牙科填充材料接触。在愈合早期阶段的人牙龈成纤维细胞(HGF-1)响应可根据用于固化牙科复合材料的程序而变化。此外，氧气扩散到牙科复合材料中抑制聚合反应，产生呈现残余未反应单体的氧抑制层(OIL)。这项研究的目的是评估不同的抛光技术和油对HGF-1的影响。
方法：采用不同技术抛光的复合圆盘（金刚石橡胶，磨料盘和碳化钨毛刺)被使用。使用具有和不具有OIL的另外的未抛光的光滑组作为对照。通过接触角测量和SEM分析其亲水性和表面形貌对样品进行物理表征，分别；然后从细胞毒性和基因表达方面分析了在不同底物上培养时HGF-1的生物反应。
结果：涂饰系统导致润湿性发生变化，即使与扩散论文的结果没有比例关系，在用一步金刚石橡胶和研磨盘抛光的表面上出现更大的增殖，以及甘油层的直接作用，证实表面粗糙度可以严重影响HGF-1的生物学响应。
结论：表面润湿性以及细胞行为似乎受到用于最终成形修复体的整理系统的选择的影响。尤其是,油的存在在人牙龈成纤维细胞方面起负面因素的作用。当修复体直接与软组织细胞接触时，本研究可能会提供有关复合材料最佳抛光系统的第一份临床指导。了解HGF-1的行为可以帮助确定抛光治疗以直接恢复与牙龈凹陷相关的龋齿/非龋齿宫颈病变。
方法：采用不同技术抛光的复合圆盘（金刚石橡胶，磨料盘和碳化钨毛刺)被使用。使用具有和不具有OIL的另外的未抛光的光滑组作为对照。通过接触角测量和SEM分析其亲水性和表面形貌对样品进行物理表征，分别；然后从细胞毒性和基因表达方面分析了在不同底物上培养时HGF-1的生物反应。
结果：涂饰系统导致润湿性发生变化，即使与扩散论文的结果没有比例关系，在用一步金刚石橡胶和研磨盘抛光的表面上出现更大的增殖，以及甘油层的直接作用，证实表面粗糙度可以严重影响HGF-1的生物学响应。
结论：表面润湿性以及细胞行为似乎受到用于最终成形修复体的整理系统的选择的影响。尤其是,油的存在在人牙龈成纤维细胞方面起负面因素的作用。当修复体直接与软组织细胞接触时，本研究可能会提供有关复合材料最佳抛光系统的第一份临床指导。了解HGF-1的行为可以帮助确定抛光治疗以直接恢复与牙龈凹陷相关的龋齿/非龋齿宫颈病变。
