PDF(Pubmed)
Abstract：
The current practice of restoring the anatomical structure in the treatment of pelvic floor dysfunction includes implantation of synthetic sling, which carries potential complications. This study aimed to develop biological substitutes to improve tissue function using scaffolds as a support to the host cells, through formation of new tissue. Human amniotic fluid stem cells (hAFSCs) were seeded on synthetic mesh-scaffold of AlloDerm Regenerative Tissue Matrix (RTM), Poly-DL-lactico-glycolic acid (PLGA) mesh (VICRYL) and Polydioxanone (PDS) meshes. In vitro study evaluates the metabolic activity of hAFSCs seeded mesh-scaffolds. In vivo study involving Sprague-Dawley rats was performed by assigning into 7 groups of sham control with fascia operation, AlloDerm implant, PDS implant, PLGA implant, AlloDerm harvest with hAFSC (AlloDerm-SC), PDS harvest with hAFSC(PDS-SC) and PLGS harvest with hAFSC (PGLA-SC). In vitro study reveals cell viability and proliferation of hAFSC on mesh scaffolds varies between meshes, with AlloDerm growing the fastest. The biomechanical properties of tissue-mesh-complex tension strength declined over time, showing highest tension strength on week-1, deteriorated similar to control group on week-12. All hAFSC-seeded mesh provides higher tension strength, compared to without. This study shed the potential of synthetic mesh as a scaffold for hAFSC for the surgical treatment of pelvic floor dysfunction.
摘要：
目前盆底功能障碍治疗中恢复解剖结构的做法包括植入合成吊带,带来潜在的并发症。这项研究旨在开发生物替代品，以提高组织功能，使用支架作为宿主细胞的支持，通过新组织的形成。将人羊水干细胞（hAFSCs）接种在AlloDerm再生组织基质（RTM）的合成网状支架上，聚-DL-乳酸-乙醇酸(PLGA)网(VICRYL)和聚二恶烷酮(PDS)网。体外研究评估接种网状支架的hAFSC的代谢活性。将Sprague-Dawley大鼠分为7组假手术对照，进行了体内研究，AlloDerm植入物,PDS植入物,PLGA植入物,用hAFSC（AlloDerm-SC）进行AlloDerm收获，PDS收获与hAFSC(PDS-SC)和PLGS收获与hAFSC(PGLA-SC)。体外研究显示hAFSC在网状支架上的细胞活力和增殖在网状物之间变化，AlloDerm增长最快。随着时间的推移，组织网复合拉伸强度的生物力学特性下降，在第1周表现出最高的张力强度，与第12周的对照组相似。所有hAFSC种子网提供更高的张力强度，与没有相比。这项研究揭示了合成网状物作为hAFSC支架用于盆底功能障碍的手术治疗的潜力。
