Abstract：
Allergic conjunctival disease is an immune-mediated inflammatory disease of the conjunctiva. To develop clinically useful drugs, it is necessary to develop quantitative evaluation methods that reflect the clinical symptoms in experimental animal models. Allergic conjunctivitis model mice were systemically sensitised with ovalbumin (OVA) administered intraperitoneally and locally sensitised with OVA eye drops between day 14-28. Next, conjunctivitis induced by ocular administration of OVA solution to sensitised mice was evaluated based on tear volume. Additionally, we evaluated increase in tear volume induced by direct ocular instillation of histamine, compound 48/80, and carrageenan. An increase in antigen-induced tear volume was observed in the mice model. Additionally, direct instillation of histamine, compound 48/80, and carrageenan increased tear volume. Furthermore, levocabastine inhibited the increase in tear volume in antigen-induced allergic conjunctivitis and histamine- and compound 48/80-induced conjunctivitis models. In contrast, betamethasone suppressed carrageenan-induced tear volume but not histamine- or compound 48/80-induced tear volume. Histamine may be involved in increased tear volume in allergic conjunctivitis. Betamethasone is not directly involved in the action of histamine and is thought to suppress increase in tear volume. Evaluation of tear volume in a conjunctivitis mice model is highly quantitative; therefore, it is possible to evaluate drug efficacy. This is considered a useful index compared with conventional methods.
摘要：
过敏性结膜疾病是一种免疫介导的结膜炎性疾病。开发临床上有用的药物，有必要在实验动物模型中建立反映临床症状的定量评价方法。过敏性结膜炎模型小鼠用腹膜内施用的卵清蛋白(OVA)全身致敏,并在第14-28天之间用OVA滴眼剂局部致敏。接下来,通过对致敏小鼠眼部给予OVA溶液诱导的结膜炎基于泪液体积进行评估.此外,我们评估了直接眼部滴注组胺引起的泪液体积增加，化合物48/80和角叉菜胶。在小鼠模型中观察到抗原诱导的泪液体积增加。此外,直接滴注组胺，化合物48/80和角叉菜胶增加泪液体积。此外，在抗原诱导的过敏性结膜炎和组胺和化合物48/80诱导的结膜炎模型中,左卡巴斯汀抑制泪液体积的增加。相比之下,倍他米松抑制角叉菜胶诱导的泪液体积，但不抑制组胺或化合物48/80诱导的泪液体积。组胺可能与过敏性结膜炎的泪液体积增加有关。倍他米松不直接参与组胺的作用，被认为可以抑制泪液体积的增加。结膜炎小鼠模型中泪液体积的评估是高度定量的；因此，评估药物疗效是可能的。与常规方法相比,这被认为是有用的指标。
