Abstract：
Purpose: To evaluate the therapeutic efficacy of topical application of a neurokinin-1 receptor (NK1R) antagonist in a rabbit model of nonallergic ocular redness. Methods: Nonallergic ocular redness was induced in rabbits by a single, topical application of dapiparzole hydrochloride eye drops (0.5%, 1%, 2%, or 5%). The NK1R antagonist L-703,606 was topically applied to the eye at the same time of induction or 20 min after induction, and phosphate buffered saline (PBS) treatment served as the control. Superior bulbar conjunctival images were taken every 30 s for the first 2 min, followed by every 4 min for 8 min, and then every 10 min until 1 h. The severity of ocular redness was evaluated on the images using ImageJ-based ocular redness index (ORI) calculations. Results: The ORI scores were significantly increased after the application of 0.5%, 1%, 2%, or 5% dapiparzole at each time point evaluated, with the most severe redness induced by the 5% dapiprazole that led to a maximal mean increase in ORI score of 14 at 20 min post-induction and thus used for subsequent evaluation of therapeutic efficacy of NK1R antagonism. Topical L-703,606, when applied at the same time as dapiprazole induction, significantly suppressed the increase of ORI scores at all time points (∼40% decrease). Furthermore, when applied at 20 min after dapiprazole induction, L-703,606 rapidly and effectively suppressed the increase of ORI scores at 30, 40, 50, and 60 min (∼30% decrease). Conclusions: Topical blockade of NK1R effectively prevents and alleviates nonallergic ocular redness in a novel animal model.
摘要：
目的：评估局部应用神经激肽1受体（NK1R）拮抗剂在兔非过敏性眼部发红模型中的疗效。方法：通过单一方法在兔中诱发非过敏性眼部发红，局部应用盐酸达帕唑滴眼液(0.5%,1%,2%,或5%）。NK1R拮抗剂L-703,606在诱导的同时或诱导后20分钟局部应用于眼睛，和磷酸盐缓冲盐水(PBS)处理作为对照。前2分钟每30秒拍摄一次球结膜上图像，然后每4分钟，共8分钟，然后每10分钟直到1小时。使用基于ImageJ的眼部发红指数(ORI)计算在图像上评估眼部发红的严重程度。结果:应用0.5%后ORI评分显著提高,1%,2%,或在每个时间点评估5%达帕唑，5%达普拉唑诱导的最严重的发红，导致诱导后20分钟ORI评分最大平均增加14分，因此用于随后评估NK1R拮抗作用的治疗效果。局部L-703,606，当与达普拉唑诱导同时应用时，在所有时间点显著抑制了ORI分数的增加(~40%下降)。此外，当在达普拉唑诱导后20分钟施用时，L-703,606在30、40、50和60分钟迅速有效地抑制了ORI分数的增加(~30%下降)。结论：在新型动物模型中，局部阻断NK1R可有效预防和减轻非过敏性眼部发红。
