Abstract：
BACKGROUND: Dermanyssus gallinae, the poultry red mite (PRM), is a worldwide ectoparasite posing significant economic challenges in poultry farming. The extensive use of pyrethroids for PRM control has led to the emergence of pyrethroid resistance. The objective of this study is to detect the pyrethroid resistance and explore its associated point mutations in the voltage-gated sodium channel (VGSC) gene among PRM populations in China.
RESULTS: Several populations of D. gallinae, namely CJF-1, CJP-2, CJP-3, CSD-4 and CLD-5, displayed varying degrees of resistance to beta-cypermethrin compared to a susceptible field population (CBP-5). Mutations of VGSC gene in populations of PRMs associated with pyrethroid resistance were identified through sequencing its fragments IIS4-IIS5 and IIIS6. The mutations I917V, M918T/L, A924G and L925V were present in multiple populations, while no mutations were found at positions T929, I936, F1534 and F1538.
CONCLUSIONS: The present study confirmed the presence of extremely high levels of pyrethroid resistance in PRM populations in China, and for the first time detected four pyrethroid resistance mutations in the VGSC gene. Identifying pyrethroid resistance in the field population of PRM in China can be achieved through screening for VGSC gene mutations as an early detection method. Our findings underscore the importance of implementing chemical PRM control strategies based on resistance evidence, while also considering the management of acaricide resistance in the control of PRMs. © 2024 Society of Chemical Industry.
RESULTS: Several populations of D. gallinae, namely CJF-1, CJP-2, CJP-3, CSD-4 and CLD-5, displayed varying degrees of resistance to beta-cypermethrin compared to a susceptible field population (CBP-5). Mutations of VGSC gene in populations of PRMs associated with pyrethroid resistance were identified through sequencing its fragments IIS4-IIS5 and IIIS6. The mutations I917V, M918T/L, A924G and L925V were present in multiple populations, while no mutations were found at positions T929, I936, F1534 and F1538.
CONCLUSIONS: The present study confirmed the presence of extremely high levels of pyrethroid resistance in PRM populations in China, and for the first time detected four pyrethroid resistance mutations in the VGSC gene. Identifying pyrethroid resistance in the field population of PRM in China can be achieved through screening for VGSC gene mutations as an early detection method. Our findings underscore the importance of implementing chemical PRM control strategies based on resistance evidence, while also considering the management of acaricide resistance in the control of PRMs. © 2024 Society of Chemical Industry.
摘要：
背景：皮氏，家禽红螨（PRM），是一种世界性的外寄生虫，对家禽养殖带来了重大的经济挑战。拟除虫菊酯用于PRM控制的广泛使用导致了拟除虫菊酯抗性的出现。本研究的目的是在中国PRM人群中检测拟除虫菊酯抗性并探讨其在电压门控钠通道（VGSC）基因中的相关点突变。
结果：一些种属D.gallinae，与易感野外种群（CBP-5）相比，即CJF-1，CJP-2，CJP-3，CSD-4和CLD-5对β-氯氰菊酯表现出不同程度的抗性。通过对其片段IIS4-IIS5和IIIS6进行测序，鉴定了与拟除虫菊酯抗性相关的PRM种群中VGSC基因的突变。突变I917V,M918T/L,A924G和L925V存在于多个群体中，而在T929、I936、F1534和F1538位置没有发现突变。
结论：本研究证实了在中国的PRM人群中存在极高的拟除虫菊酯抗性，并首次在VGSC基因中检测到四个拟除虫菊酯抗性突变。通过筛选VGSC基因突变作为早期检测方法，可以在中国PRM的野外种群中鉴定拟除虫菊酯抗性。我们的发现强调了基于耐药性证据实施化学PRM控制策略的重要性，同时还考虑了在控制PRM中对杀螨剂抗性的管理。©2024化学工业学会。
结果：一些种属D.gallinae，与易感野外种群（CBP-5）相比，即CJF-1，CJP-2，CJP-3，CSD-4和CLD-5对β-氯氰菊酯表现出不同程度的抗性。通过对其片段IIS4-IIS5和IIIS6进行测序，鉴定了与拟除虫菊酯抗性相关的PRM种群中VGSC基因的突变。突变I917V,M918T/L,A924G和L925V存在于多个群体中，而在T929、I936、F1534和F1538位置没有发现突变。
结论：本研究证实了在中国的PRM人群中存在极高的拟除虫菊酯抗性，并首次在VGSC基因中检测到四个拟除虫菊酯抗性突变。通过筛选VGSC基因突变作为早期检测方法，可以在中国PRM的野外种群中鉴定拟除虫菊酯抗性。我们的发现强调了基于耐药性证据实施化学PRM控制策略的重要性，同时还考虑了在控制PRM中对杀螨剂抗性的管理。©2024化学工业学会。
