Abstract：
Importance of Study: Semen cryopreservation results in sperm damage due to lipid peroxidation or oxidative stress, leading to a decrease in conception rate. The sperm damage during cryopreservation can be minimized with the use of suitable antioxidant supplements in semen diluent. Some herbs have potent antioxidant potential and can be used in semen diluent to protect the spermatozoa. Objective: Hence, the investigation was planned to evaluate the effect of Asparagus racemosus (A. racemosus) aqueous extract on buck semen quality during cryopreservation. Methodology: In the current study, semen was collected from eight Sirohi bucks, and from each buck, 8 ejaculates were collected. Good-quality semen samples were pooled during each collection. Pooled semen samples were then divided into four equal parts and diluted in TRIS buffer containing different concentrations of A. racemosus aqueous extract (different groups, i.e., G I -5 mg, G II -2.5 mg, G III -1.25 mg, and G IV -0 mg of A. racemosus aqueous extract in 1 mL TRIS buffer). All the diluted semen samples were kept at equilibration temperature (5°C) for 2 hours and then cryopreserved by the manual method. Semen samples were evaluated for various sperm characteristics and antioxidant status before and after cryopreservation. Results: Asparagus racemosus aqueous extract showed significant (p < 0.05) enhancement of sperm viability, sperm motility, acrosomal integrity, and plasma membrane integrity, whereas it reduced sperm abnormality. Furthermore, in the experimental groups, the antioxidant gene expression was found to be increased compared to that of the treatment group. G III (p < 0.05) showed significantly better results in terms of sperm viability, sperm motility, acrosomal integrity, and plasma membrane integrity. Conclusion: Asparagus racemosus aqueous extract has the antioxidant potential to protect buck spermatozoa during semen cryopreservation.
摘要：
研究的重要性：由于脂质过氧化或氧化应激，精液冷冻保存导致精子损伤，导致受胎率下降。在精液稀释剂中使用合适的抗氧化剂补充剂可以使冷冻保存期间的精子损伤最小化。一些草药具有有效的抗氧化潜力，可用于精液稀释剂以保护精子。目标：因此，这项调查计划评估芦笋的效果(A.racemosus）冷冻保存期间水提物对雄鹿精液质量的影响。方法：在目前的研究中，精液是从八块Sirohi美元收集的，从每一块钱中，收集了8个射精。在每次收集期间收集高质量的精液样品。然后将收集的精液样品分为四等份，并在含有不同浓度的曲柳水提取物的TRIS缓冲液中稀释（不同组，即，GI-5mg,GII-2.5mg，GIII-1.25mg，和GIV-Omg在1mLTRIS缓冲液中的A.racemosus水提取物)。将所有稀释的精液样品在平衡温度(5°C)下保持2小时,然后通过手动方法冷冻保存。在冷冻保存之前和之后评估精液样品的各种精子特征和抗氧化剂状态。结果:芦笋水提物显著(p<0.05)提高精子活力,精子运动性,顶体完整性,和质膜的完整性，而它减少了精子异常。此外，在实验组中，与治疗组相比，抗氧化基因表达增加。GIII（p<0.05）在精子活力方面显示出明显更好的结果，精子运动性,顶体完整性,和质膜完整性。结论:芦笋水提物在精液冷冻保存过程中具有一定的抗氧化保护作用。
