PDF(Pubmed)
Abstract：
UNASSIGNED: Aedes albopictus, like Aedes aegypti, is a virulent vector of arboviruses especially the well-documented spread of yellow fever around the world. Although yellow fever is prevalent in Nigeria, there is a paucity of information in the Niger Delta region on the distribution of Aedes mosquito vectors and molecular detection of the virus in infected mosquitoes. This study sampled Aedes mosquitoes around houses associated with farms from four communities (Otolokpo, Ute-Okpu, Umunede, and Ute Alohen) in Ika North-East Local Government Area of Delta State, Nigeria.
UNASSIGNED: various sampling methods were used in Aedes mosquito collection to test their efficacy in the survey. Mosquitoes in holding cages were killed by freezing and morphologically identified. A pool of 15 mosquitoes per Eppendorf tube was preserved in RNAi later for yellow fever virus screening. Two samples were molecularly screened for each location.
UNASSIGNED: seven hundred and twenty-five (725) mosquitoes were obtained from the various traps. The mean abundance of the mosquitoes was highest in m-HLC (42.9) compared to the mosquitoes sampled using other techniques (p<0.0001). The mean abundance of mosquitoes was lowest in Center for Disease Control (CDC) light traps without attractant (0.29). No yellow fever virus strain was detected in all the mosquitoes sampled at the four locations.
UNASSIGNED: this study suggests that Aedes albopictus are the mosquitoes commonly biting around houses associated with farms. More so, yellow fever virus was not detected in the mosquitoes probably due to the mass vaccination exercise that was carried out the previous year in the study area. More studies are required using the m-HLC to determine the infection rate in this endemic area.
UNASSIGNED: various sampling methods were used in Aedes mosquito collection to test their efficacy in the survey. Mosquitoes in holding cages were killed by freezing and morphologically identified. A pool of 15 mosquitoes per Eppendorf tube was preserved in RNAi later for yellow fever virus screening. Two samples were molecularly screened for each location.
UNASSIGNED: seven hundred and twenty-five (725) mosquitoes were obtained from the various traps. The mean abundance of the mosquitoes was highest in m-HLC (42.9) compared to the mosquitoes sampled using other techniques (p<0.0001). The mean abundance of mosquitoes was lowest in Center for Disease Control (CDC) light traps without attractant (0.29). No yellow fever virus strain was detected in all the mosquitoes sampled at the four locations.
UNASSIGNED: this study suggests that Aedes albopictus are the mosquitoes commonly biting around houses associated with farms. More so, yellow fever virus was not detected in the mosquitoes probably due to the mass vaccination exercise that was carried out the previous year in the study area. More studies are required using the m-HLC to determine the infection rate in this endemic area.
摘要：
■白纹伊蚊，比如埃及伊蚊,是虫媒病毒的毒力载体，尤其是黄热病在世界各地的传播有据可查。尽管黄热病在尼日利亚很普遍，在尼日尔三角洲地区，关于伊蚊媒介的分布和感染蚊子中病毒的分子检测的信息很少。这项研究对来自四个社区（Otolokpo，Ute-Okpu,Umunede,和UteAlohen）位于三角洲州伊卡东北地方政府区，尼日利亚。
■在伊蚊收集中使用了各种采样方法，以测试其在调查中的功效。固定笼中的蚊子通过冷冻杀死并在形态上进行鉴定。每个Eppendorf管的15只蚊子池随后在RNAi中保存用于黄热病病毒筛选。对每个位置的两个样品进行分子筛选。
■从各种陷阱中获得了七百二十五(725)只蚊子。与使用其他技术取样的蚊子相比,在m-HLC中蚊子的平均丰度最高(42.9)(p<0.0001)。在没有引诱剂的疾病控制中心(CDC)光阱中,蚊子的平均丰度最低(0.29)。在四个地点采样的所有蚊子中均未检测到黄热病病毒株。
■这项研究表明，白纹伊蚊是通常在与农场相关的房屋周围叮咬的蚊子。更多，在蚊子中没有检测到黄热病病毒，可能是由于前一年在研究区域进行了大规模疫苗接种活动。需要使用m-HLC进行更多研究以确定该流行地区的感染率。
■在伊蚊收集中使用了各种采样方法，以测试其在调查中的功效。固定笼中的蚊子通过冷冻杀死并在形态上进行鉴定。每个Eppendorf管的15只蚊子池随后在RNAi中保存用于黄热病病毒筛选。对每个位置的两个样品进行分子筛选。
■从各种陷阱中获得了七百二十五(725)只蚊子。与使用其他技术取样的蚊子相比,在m-HLC中蚊子的平均丰度最高(42.9)(p<0.0001)。在没有引诱剂的疾病控制中心(CDC)光阱中,蚊子的平均丰度最低(0.29)。在四个地点采样的所有蚊子中均未检测到黄热病病毒株。
■这项研究表明，白纹伊蚊是通常在与农场相关的房屋周围叮咬的蚊子。更多，在蚊子中没有检测到黄热病病毒，可能是由于前一年在研究区域进行了大规模疫苗接种活动。需要使用m-HLC进行更多研究以确定该流行地区的感染率。
