UNASSIGNED: ONC was induced in the left eye of mice by short-term clamping of the optic nerve; oral artemisinin was administered daily. The neuroprotective effect of the drug was assessed using Tuj-1 staining in RGCs. In addition, the inflammatory response and the expression levels of phosphorylated tau protein and tau oligomers were observed using RT-qPCR, TUNEL assay, and fluorescence staining to investigate the underlying mechanisms.
UNASSIGNED: Artemisinin increased the survival rate of RGCs 14 days after ONC. Artemisinin significantly reduced the levels of inflammatory factors such as CXCL10, CXCR3, and IL-1β in the retina and decreased the apoptosis of RGCs. Moreover, downregulation of the phosphorylation of tau proteins and the expression of tau oligomers were observed after artemisinin treatment.
UNASSIGNED: Our results suggest that artemisinin can increase the survival rate of RGCs after ONC and reduce their apoptosis. This effect may be achieved by inhibiting the inflammatory response it triggers and downregulating tau protein phosphorylation and tau oligomer expression. These findings suggest the potential application of artemisinin as a therapeutic agent for neuropathy.
■通过短期夹住视神经在小鼠的左眼中诱导ONC;每天口服青蒿素。在RGC中使用Tuj-1染色评估药物的神经保护作用。此外,用RT-qPCR观察炎症反应和磷酸化tau蛋白和tau寡聚体的表达水平,TUNEL检测,和荧光染色,以探讨潜在的机制。
■青蒿素在ONC后14天增加了RGC的存活率。青蒿素能显著降低视网膜中CXCL10、CXCR3、IL-1β等炎症因子的水平,降低RGCs的凋亡。此外,青蒿素处理后,观察到tau蛋白磷酸化和tau寡聚体表达的下调。
■我们的结果表明,青蒿素可以提高ONC后RGC的存活率并减少其凋亡。这种效果可以通过抑制其引发的炎症反应并下调tau蛋白磷酸化和tau寡聚体表达来实现。这些发现表明青蒿素作为神经病的治疗剂的潜在应用。