PDF(Pubmed)
Abstract：
UNASSIGNED: To evaluate a PCR based method of polyacrylamide gel electrophoresis of short tandem repeats and its quantification for detecting donor chimerism after haematopoietic stem cell transplantation in acute leukaemias.
UNASSIGNED: The descriptive study was conducted at Genetic Resource Centre (GRC) Lab Rawalpindi from Feb 2018 - Nov 2020. A total of twenty patients with acute leukaemias having undergone HSCT were selected and assessed for the analysis of chimerism status. DNA extraction from the whole blood was done by chelex method and short tandem repeats were amplified by using conventional STR- PCR assay. Electrophoresis was carried out and 6% polyacrylamide gels were used for the resultant amplified DNA products and then followed by their densitometry. These patients had undergone HSCT from Pakistan Institute of Medical Science and Armed Forces Bone Marrow Transplant Centre.
UNASSIGNED: The peaks in the PAGE densitometry represented the donor chimerism in all post transplant samples of the patients.
UNASSIGNED: Our study showed that densitometry of STR PCR PAGE is a useful and cheaper method for demonstration of donor chimerism in acute leukaemia patients having undergone HSCT. Hence this method can be a valuable option in the monitoring of chimerism status in these patients and therefore helps in preventing graft failure by fast and early treatment strategies for these patients.
UNASSIGNED: The descriptive study was conducted at Genetic Resource Centre (GRC) Lab Rawalpindi from Feb 2018 - Nov 2020. A total of twenty patients with acute leukaemias having undergone HSCT were selected and assessed for the analysis of chimerism status. DNA extraction from the whole blood was done by chelex method and short tandem repeats were amplified by using conventional STR- PCR assay. Electrophoresis was carried out and 6% polyacrylamide gels were used for the resultant amplified DNA products and then followed by their densitometry. These patients had undergone HSCT from Pakistan Institute of Medical Science and Armed Forces Bone Marrow Transplant Centre.
UNASSIGNED: The peaks in the PAGE densitometry represented the donor chimerism in all post transplant samples of the patients.
UNASSIGNED: Our study showed that densitometry of STR PCR PAGE is a useful and cheaper method for demonstration of donor chimerism in acute leukaemia patients having undergone HSCT. Hence this method can be a valuable option in the monitoring of chimerism status in these patients and therefore helps in preventing graft failure by fast and early treatment strategies for these patients.
摘要：
■评估基于PCR的短串联重复的聚丙烯酰胺凝胶电泳方法及其定量，以检测急性白血病中造血干细胞移植后的供体嵌合状态。
■描述性研究于2018年2月至2020年11月在遗传资源中心（GRC）拉瓦尔品第实验室进行。选择并评估了总共20例接受HSCT的急性白血病患者，以分析嵌合状态。通过chelex方法从全血中提取DNA，并通过使用常规STR-PCR测定法扩增短串联重复序列。进行电泳并将6%聚丙烯酰胺凝胶用于所得扩增的DNA产物,然后进行其密度测定。这些患者接受了巴基斯坦医学科学研究所和武装部队骨髓移植中心的HSCT。
■PAGE密度测定中的峰表示患者的所有移植后样品中的供体嵌合状态。
■我们的研究表明，STRPCRPAGE的密度测定是证明接受HSCT的急性白血病患者供体嵌合状态的一种有用且便宜的方法。因此,该方法可以是监测这些患者的嵌合状态的有价值的选择,因此有助于通过对这些患者的快速和早期治疗策略来预防移植物衰竭。
■描述性研究于2018年2月至2020年11月在遗传资源中心（GRC）拉瓦尔品第实验室进行。选择并评估了总共20例接受HSCT的急性白血病患者，以分析嵌合状态。通过chelex方法从全血中提取DNA，并通过使用常规STR-PCR测定法扩增短串联重复序列。进行电泳并将6%聚丙烯酰胺凝胶用于所得扩增的DNA产物,然后进行其密度测定。这些患者接受了巴基斯坦医学科学研究所和武装部队骨髓移植中心的HSCT。
■PAGE密度测定中的峰表示患者的所有移植后样品中的供体嵌合状态。
■我们的研究表明，STRPCRPAGE的密度测定是证明接受HSCT的急性白血病患者供体嵌合状态的一种有用且便宜的方法。因此,该方法可以是监测这些患者的嵌合状态的有价值的选择,因此有助于通过对这些患者的快速和早期治疗策略来预防移植物衰竭。
