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Abstract：
Circular RNAs (circRNAs) have been recognized as pivotal regulators in tumorigenesis, yet the biological functions as well as molecular mechanisms of the majority of circRNAs in hepatocellular carcinoma (HCC) remain elusive. We sought to unveil the expression profile and biological role of circMYBL2 in HCC. Initial microarray analyses were conducted to probe the expression profile of circMYBL2 in HCC cells, and qRT‒PCR analysis was then performed in HCC cell lines and tissues, revealing significant upregulation of circMYBL2. Subsequent experiments were conducted to evaluate the biological function of circMYBL2 in HCC progression. Furthermore, bioinformatics analysis, qRT‒PCR analysis, luciferase reporter assays, and western blot analysis were employed to investigate the interplay among circMYBL2, miR-1205, and E2F1. CircMYBL2 was found to exhibit marked upregulation in tumor tissues as well as HCC cell lines. Elevated expression of circMYBL2 increased the proliferation and migration of HCC cells, whereas circMYBL2 knockdown elicited contrasting effects. Mechanistically, our results indicated that circMYBL2 promoted E2F1 expression and facilitated HCC progression by sponging miR-1205. Our findings revealed that circMYBL2 contributed to HCC progression through the circMYBL2/miR-1205/E2F1 axis, suggesting the potential of circMYBL2 as a novel target for HCC treatment or a prognostic biomarker for HCC.
摘要：
环状RNA（circularRNAs）已被认为是肿瘤发生中的关键调节因子，然而，大多数circRNAs在肝细胞癌（HCC）中的生物学功能和分子机制仍然难以捉摸。我们试图揭示circMYBL2在HCC中的表达谱和生物学作用。进行初始微阵列分析以探测HCC细胞中circMYBL2的表达谱，然后在HCC细胞系和组织中进行qRT-PCR分析，揭示了大约MYBL2的显著上调。随后进行实验以评估circMYBL2在HCC进展中的生物学功能。此外，生物信息学分析，qRT-PCR分析，荧光素酶报告基因测定,和蛋白质印迹分析用于研究circMYBL2、miR-1205和E2F1之间的相互作用。发现CircMYBL2在肿瘤组织和HCC细胞系中表现出明显的上调。circMYBL2的高表达增加了肝癌细胞的增殖和迁移,而大约MYBL2敲除会产生相反的效果。机械上,我们的结果表明,circMYBL2促进E2F1表达,并通过生成miR-1205促进HCC进展.我们的发现显示，circMYBL2通过circMYBL2/miR-1205/E2F1轴促进HCC进展，提示circMYBL2作为HCC治疗的新靶点或HCC的预后生物标志物的潜力。
