PDF(Pubmed)
Abstract：
Lactobacillus spp. inhibit the growth of Campylobacter spp. in vitro. However, in chicken crops, in which Lactobacillus spp. predominate, such inhibition of Campylobacter has not been confirmed. In our previous study, feeding paddy rice to broiler chicks increased the residence time of the food, which might enhance the bactericidal activity of the crop. Here, the bactericidal activity against the remaining Campylobacter spp. in broiler crops was evaluated. A suspension prepared by mixing Campylobacter jejuni and titanium dioxide (TiO2) was inoculated into the pharynx of 26-day-old broiler chicks fed a paddy rice-based diet. The crop contents were sampled at 20-min intervals. The TiO2 residual ratio in the crop gradually decreased with time after inoculation, with 57% of the inoculated TiO2 remaining in the crop 60 min after inoculation. The survival fraction of C. jejuni in the crops was 11% at 40 min, only 1% at 60 min, and was undetectable at 80 min. Most of the inoculated C. jejuni died in the crop before entering the next segment. These data indicated that bacterial death occurred between 30 min and 40 min after inoculation. The average survival time of C. jejuni in the crop was calculated to be 37.1 min. Thus, C. jejuni remaining in a chicken crop for more than 40 min died.
摘要：
乳杆菌属。抑制弯曲杆菌的生长。在体外。然而,在鸡肉作物中，其中乳酸菌属。占主导地位，这种对弯曲杆菌的抑制作用尚未得到证实。在我们之前的研究中,将水稻饲喂给肉鸡增加了食物的停留时间，这可能会增强作物的杀菌活性。这里,对剩余弯曲杆菌属的杀菌活性。在肉鸡作物中进行了评估。将通过混合空肠弯曲杆菌和二氧化钛（TiO2）制备的悬浮液接种到以稻米为基础的饮食喂养的26天大肉鸡的咽部。每隔20分钟对作物内容物进行采样。接种后随着时间的延长,作物中TiO2的残留率逐渐降低,在接种后60分钟,57%的接种的TiO2保留在作物中。空肠弯曲菌在40分钟时的成活率为11％，60分钟时只有1%，并且在80分钟时检测不到。大多数接种的空肠弯曲杆菌在进入下一段之前在作物中死亡。这些数据表明细菌死亡发生在接种后30分钟至40分钟之间。计算空肠弯曲菌在作物中的平均存活时间为37.1分钟。因此，在鸡作物中停留超过40分钟的空肠杆菌死亡。
