PDF(Pubmed)
Abstract：
UNASSIGNED: Histones have been associated with human diseases. However, the implication of extranuclear histone proteins and their potential mechanism in the pathophysiology of chronic rhinosinusitis (CRS) have not been thoroughly investigated. This study was designed to evaluate the role of histones in patients with CRS by comparing histone expression between patients and controls.
UNASSIGNED: Nasal polyp (NP) tissues were obtained, and their comprehensive gene expression profiles were investigated by microarray analysis. Differences in expression were verified by reverse transcriptase polymerase chain reaction and immunohistochemical staining. Cell culture and flow cytometry were used to evaluate the role of histones in the pathogenesis of polyps.
UNASSIGNED: Significant differences in the microarray analysis were observed between the patient and control groups (P < 0.01). It was found by flow cytometry that the histone (H2BK) can promote cell apoptosis in NPs.
UNASSIGNED: Our results indicate that reduced expression of H2BK may contribute to the imbalance process of cell proliferation and apoptosis in CRS with NP.
UNASSIGNED: Nasal polyp (NP) tissues were obtained, and their comprehensive gene expression profiles were investigated by microarray analysis. Differences in expression were verified by reverse transcriptase polymerase chain reaction and immunohistochemical staining. Cell culture and flow cytometry were used to evaluate the role of histones in the pathogenesis of polyps.
UNASSIGNED: Significant differences in the microarray analysis were observed between the patient and control groups (P < 0.01). It was found by flow cytometry that the histone (H2BK) can promote cell apoptosis in NPs.
UNASSIGNED: Our results indicate that reduced expression of H2BK may contribute to the imbalance process of cell proliferation and apoptosis in CRS with NP.
摘要：
■组蛋白与人类疾病有关。然而,核外组蛋白在慢性鼻-鼻窦炎(CRS)病理生理学中的意义及其潜在机制尚未得到彻底研究.这项研究旨在通过比较患者和对照组之间的组蛋白表达来评估组蛋白在CRS患者中的作用。
■获得鼻息肉（NP）组织，并通过微阵列分析研究了它们的综合基因表达谱。通过逆转录聚合酶链反应和免疫组织化学染色验证表达的差异。细胞培养和流式细胞术用于评估组蛋白在息肉发病机理中的作用。
■在患者组和对照组之间观察到微阵列分析的显着差异（P<0.01）。通过流式细胞术发现，组蛋白（H2BK）可以促进NPs中的细胞凋亡。
■我们的结果表明，H2BK的表达降低可能有助于NP在CRS中细胞增殖和凋亡的失衡过程。
■获得鼻息肉（NP）组织，并通过微阵列分析研究了它们的综合基因表达谱。通过逆转录聚合酶链反应和免疫组织化学染色验证表达的差异。细胞培养和流式细胞术用于评估组蛋白在息肉发病机理中的作用。
■在患者组和对照组之间观察到微阵列分析的显着差异（P<0.01）。通过流式细胞术发现，组蛋白（H2BK）可以促进NPs中的细胞凋亡。
■我们的结果表明，H2BK的表达降低可能有助于NP在CRS中细胞增殖和凋亡的失衡过程。
