Abstract：
Fluorescent nanosensors have revolutionized diagnostics and our ability to monitor cellular dynamics. Yet, distinguishing sensor signals from autofluorescence remains a challenge. Here, we merged optode-based sensing with near-infrared-emitting ZnGa2O4:Cr3+ persistent luminescence nanoparticles (PLNPs) to create nanocomposites for autofluorescence-free \"glow-in-the-dark\" sensing. Hydrophobic modification and incorporation of the persistent luminescence nanoparticles into an optode-based nanoparticle core yielded persistent luminescence nanosensors (PLNs) for five analytes (K+, Na+, Ca2+, pH, and O2) via two distinct mechanisms. We demonstrated the viability of the PLNs by quantifying K+ in fetal bovine serum, calibrating the pH PLNs in the same, and ratiometrically monitoring O2 metabolism in cultures of Saccharomyces cerevisiae, all the while overcoming their respective autofluorescence signatures. This highly modular platform allows for facile tuning of the sensing functionality, optical properties, and surface chemistry and promises high signal-to-noise ratios in complex optical environments.
摘要：
荧光纳米传感器彻底改变了诊断和我们监测细胞动力学的能力。然而，区分传感器信号从自发荧光仍然是一个挑战。这里,我们将基于光电探测器的传感与近红外发射的ZnGa2O4:Cr3+持久发光纳米粒子(PLNPs)结合起来,形成了用于无自发荧光“暗光发光”传感的纳米复合材料。疏水修饰并将持久性发光纳米颗粒掺入基于光电极的纳米颗粒核心中，产生了用于五种分析物（K，Na+,Ca2+,pH值,和O2）通过两种不同的机制。我们通过定量胎牛血清中的K来证明PLN的生存能力，校准相同的pH值PLN，并按比例监测酿酒酵母培养物中的O2代谢，同时克服他们各自的自发荧光特征。这个高度模块化的平台允许轻松调整传感功能，光学性质,和表面化学和承诺在复杂的光学环境高信噪比。
