Abstract：
BACKGROUND: Breast cancer (BC) is associated with a continuous increase in incidence, with high mortality rates in several countries. CD44, STAT3, and SOX2 are related to regulating of somatic cell division, tumorigenesis, and metastasis in BC.
METHODS: A cross-sectional study was carried out at the Hospital de Cancer de Pernambuco (HCP) between 2017 and 2018. Fifty-one women with locally advanced (LA) and 14 with metastatic BC were included in the study.
RESULTS: High CD44+/CD24neg and CD44+/CD24neg/SOX2+ levels in Luminal B (LB), HER2+, and triple-negative breast cancer (TNBC) compared with controls (p < 0.05). Low CD44+/CD24negSTAT3+ levels in LB, HER2+, and TNBC compared with controls (p < 0.05). High T lymphocytes, and low STAT3 + T, and SOX2 + T levels in BC patients (p < 0.05). High SOX2 + T levels in patients with axillary lymph node-negative (N0) compared with the axillary lymph node-positives (N1 and N2 groups; p < 0.05). High SOX2 + T levels in N1 compared to N2 (p < 0.05). High T lymphocytes and low SOX2 + T levels in the LA tumor compared to metastatic disease (p = 0.0007 and p = 0.02, respectively). High CD44 + /CD24negSTAT3+, and T lymphocyte levels in TNBC patients with LA tumor compared to metastatic (p < 0.05). Low STAT3 + T levels in TBNC patients with LA tumor compared to metastatic (p = 0.0266).
CONCLUSIONS: SOX2 and STAT3 expression on circulating T lymphocytes and CD44 + /CD24neg cells in peripheral blood have prognostic roles in breast cancer. SOX2 and STAT3 expression are potential predictive biomarkers of disease progression in breast cancer regardless of tumor subtype.
METHODS: A cross-sectional study was carried out at the Hospital de Cancer de Pernambuco (HCP) between 2017 and 2018. Fifty-one women with locally advanced (LA) and 14 with metastatic BC were included in the study.
RESULTS: High CD44+/CD24neg and CD44+/CD24neg/SOX2+ levels in Luminal B (LB), HER2+, and triple-negative breast cancer (TNBC) compared with controls (p < 0.05). Low CD44+/CD24negSTAT3+ levels in LB, HER2+, and TNBC compared with controls (p < 0.05). High T lymphocytes, and low STAT3 + T, and SOX2 + T levels in BC patients (p < 0.05). High SOX2 + T levels in patients with axillary lymph node-negative (N0) compared with the axillary lymph node-positives (N1 and N2 groups; p < 0.05). High SOX2 + T levels in N1 compared to N2 (p < 0.05). High T lymphocytes and low SOX2 + T levels in the LA tumor compared to metastatic disease (p = 0.0007 and p = 0.02, respectively). High CD44 + /CD24negSTAT3+, and T lymphocyte levels in TNBC patients with LA tumor compared to metastatic (p < 0.05). Low STAT3 + T levels in TBNC patients with LA tumor compared to metastatic (p = 0.0266).
CONCLUSIONS: SOX2 and STAT3 expression on circulating T lymphocytes and CD44 + /CD24neg cells in peripheral blood have prognostic roles in breast cancer. SOX2 and STAT3 expression are potential predictive biomarkers of disease progression in breast cancer regardless of tumor subtype.
摘要：
背景：乳腺癌（BC）与发病率的持续增加有关，几个国家的死亡率很高。CD44，STAT3和SOX2与体细胞分裂的调节有关，肿瘤发生，和BC中的转移。
方法：2017年至2018年在伯南布哥州癌症医院（HCP）进行了一项横断面研究。51名局部晚期（LA）妇女和14名转移性BC妇女被纳入研究。
结果：管腔B（LB）中高CD44/CD24neg和CD44/CD24neg/SOX2水平，HER2+,和三阴性乳腺癌（TNBC）与对照组相比（p<0.05）。LB中CD44+/CD24negSTAT3+水平低，HER2+,和TNBC与对照组相比(p<0.05)。高T淋巴细胞，和低STAT3+T，BC患者的SOX2+T水平(p<0.05)。腋窝淋巴结阴性(N0)患者SOX2+T水平高于腋窝淋巴结阳性(N1和N2组,p<0.05)。与N2相比,N1中的高SOX2+T水平(p<0.05)。与转移性疾病相比，LA肿瘤中的高T淋巴细胞和低SOX2T水平（分别为p=0.0007和p=0.02）。高CD44+/CD24negSTAT3+,与转移性肿瘤的TNBC患者的T淋巴细胞水平相比（p<0.05）。与转移性相比，患有LA肿瘤的TBNC患者的STAT3T水平较低（p=0.0266）。
结论：外周血中循环T淋巴细胞和CD44+/CD24neg细胞中SOX2和STAT3的表达与乳腺癌的预后有关。SOX2和STAT3表达是乳腺癌疾病进展的潜在预测生物标志物，与肿瘤亚型无关。
方法：2017年至2018年在伯南布哥州癌症医院（HCP）进行了一项横断面研究。51名局部晚期（LA）妇女和14名转移性BC妇女被纳入研究。
结果：管腔B（LB）中高CD44/CD24neg和CD44/CD24neg/SOX2水平，HER2+,和三阴性乳腺癌（TNBC）与对照组相比（p<0.05）。LB中CD44+/CD24negSTAT3+水平低，HER2+,和TNBC与对照组相比(p<0.05)。高T淋巴细胞，和低STAT3+T，BC患者的SOX2+T水平(p<0.05)。腋窝淋巴结阴性(N0)患者SOX2+T水平高于腋窝淋巴结阳性(N1和N2组,p<0.05)。与N2相比,N1中的高SOX2+T水平(p<0.05)。与转移性疾病相比，LA肿瘤中的高T淋巴细胞和低SOX2T水平（分别为p=0.0007和p=0.02）。高CD44+/CD24negSTAT3+,与转移性肿瘤的TNBC患者的T淋巴细胞水平相比（p<0.05）。与转移性相比，患有LA肿瘤的TBNC患者的STAT3T水平较低（p=0.0266）。
结论：外周血中循环T淋巴细胞和CD44+/CD24neg细胞中SOX2和STAT3的表达与乳腺癌的预后有关。SOX2和STAT3表达是乳腺癌疾病进展的潜在预测生物标志物，与肿瘤亚型无关。
