Abstract：
This study aims to investigate the influence of thymol on primordial follicle growth and survival, as well as on collagen fibers and stromal cells density in bovine ovarian tissues cultured in vitro. The activity of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX), the thiol levels and the expression of mRNAs for SOD1, CAT, periredoxin 6 (PRDX6) and GPX1 were also investigated. Ovarian cortical tissues were cultured in α-MEM+ alone or with thymol (400, 800, 1600 or 3200 μg/mL) for six days. Before and after culture, the tissues were processed for histological analysis to evaluate follicular activation, growth, morphology, ovarian stromal cell density and collagen fibers. The levels of mRNA for SOD1, CAT, GPX1 and PRDX6 were evaluated by real-time PCR. The results show that tissues cultured with thymol (400 and 800 µg/mL) had increased percentages of normal follicles, when compared to tissues cultured in other treatments. At concentrations of 400 and 800 µg/mL, thymol maintained the rate of normal follicles similar to the uncultured control. In addition, 400 µg/mL thymol increased follicle activation, collagen fibers and stromal cell density of when compared to tissues cultured in control medium. The presence of 800 µg/mL thymol in culture medium increased CAT activity, while 400 or 800 µg/mL thymol reduced mRNA levels for SOD1, CAT and PRDX6, but did not alter GPX1 expression. In conclusion, 400 µg/mL thymol increases primordial follicle activation, preserves stromal cells, collagen fibers, and down-regulates expression of mRNA for SOD1, CAT and PRDX6 in cultured bovine ovarian tissues.
摘要：
本研究旨在探讨百里酚对原始卵泡生长和存活的影响,以及体外培养的牛卵巢组织中胶原纤维和基质细胞密度。超氧化物歧化酶（SOD）的活性，过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GPX），SOD1，CAT的硫醇水平和mRNA的表达，还研究了周效还蛋白6（PRDX6）和GPX1。将卵巢皮质组织在α-MEM+中单独培养或与百里酚(400、800、1600或3200μg/mL)一起培养6天。文化之前和之后，对组织进行组织学分析以评估卵泡激活，增长,形态学,卵巢基质细胞密度和胶原纤维。SOD1、CAT、通过实时PCR评估GPX1和PRDX6。结果表明，用百里酚（400和800µg/mL）培养的组织中正常卵泡的百分比增加，与其他处理中培养的组织相比。在400和800微克/毫升的浓度下，百里酚维持正常卵泡的比率与未培养的对照相似。此外,400微克/毫升百里酚增加卵泡激活，与在对照培养基中培养的组织相比，胶原纤维和基质细胞密度。培养基中800μg/mL百里酚的存在增加了CAT活性，而400或800µg/mL百里酚降低了SOD1，CAT和PRDX6的mRNA水平，但没有改变GPX1的表达。总之,400微克/毫升百里酚增加原始卵泡激活，保留基质细胞，胶原纤维，并下调培养的牛卵巢组织中SOD1，CAT和PRDX6的mRNA表达。
