Abstract：
In our research we explore the world of PACDs, carbon dots synthesized from pamoic acid through a single step pyrolysis method. Our findings reveal that PACDs have capabilities of serving as sensitive and selective sensors in both colorimetric and fluorescent modes. They are particularly effective, at colorimetrically and fluorometrically detecting ferric ions and can also act as fluorometric sensors for pH. When ferric ions are introduced an interesting transformation occurs. A noticeable change in color unfolds before our eyes, under 365 nm UV light the fluorescence shifts from green to blue while in daylight it changes from a yellow to a deep ink blue. Notably these detection techniques can precisely measure ferric ions within concentrations ranging from 5 µM to 80 µM with a detection limit of 0.1 µM for fluorescence response. Additionally, they can detect ferric ions colorimetrically within the range of 5 µM to 45 µM with a detection limit of 3.8 µM. Furthermore, the PACDs exhibit a capability to adapt to different pH levels. In alkaline environments with a pH range between 8 and 11 the fluorescence signal demonstrates a response that directly correlates with pH levels and slightly shifts its position. In contrast under acidic conditions a noticeable shift, towards blue is observed in the fluorescence signal leading to a change in color from green to blue when exposed to UV light. This shift persists as the fluorescence signal directly correlates with decreasing pH levels in settings. Apart from their proficiency in ferric ion detection and pH monitoring, the PACDs also demonstrate potential in cancer research. Through an assessment using the MTT assay it was discovered that the PACDs exhibit cytotoxic effects against five different cancer cell lines; HCT 116, MDA MB 231, Hep3B, MCF 7 and HeLa. The findings are promising as the PACDs show IC50 values of 12.5 µg/ml 6.25 µg/ml 25 µg/ml 50 µg/ml and 100 µg/ml for these cell lines. This research highlights the versatility and potential of PACDs as a tool, in both sensing applications and oncology research.
摘要：
在我们的研究中，我们探索了PACD的世界，通过单步骤热解方法由双羟甲基苯甲酸合成的碳点。我们的发现表明，PACD具有在比色和荧光模式下充当灵敏和选择性传感器的能力。它们特别有效，在比色法和荧光法上检测三价铁离子，也可以用作pH值的荧光传感器。当引入三价铁离子时，会发生有趣的转化。明显的颜色变化展现在我们眼前，在365nm紫外光下,荧光从绿色转变为蓝色,而在日光下,荧光从黄色转变为深墨蓝。值得注意的是，这些检测技术可以精确测量浓度范围为5µM至80µM的三价铁离子，荧光响应的检测极限为0.1µM。此外,它们可以比色检测5µM至45µM范围内的三价铁离子，检出限为3.8µM。此外，PACD表现出适应不同pH水平的能力。在pH范围在8和11之间的碱性环境中,荧光信号表明与pH水平直接相关并稍微改变其位置的响应。相比之下，在酸性条件下有明显的变化，在暴露于UV光时,在荧光信号中观察到朝向蓝色的颜色从绿色变为蓝色。由于荧光信号与设置中降低的pH水平直接相关，因此这种偏移持续存在。除了精通三价铁离子检测和pH监测，PACDs也显示出在癌症研究中的潜力。通过使用MTT测定的评估，发现PACD对五种不同的癌细胞系表现出细胞毒性作用;HCT116，MDAMB231，Hep3B，MCF7和HeLa。这些发现是有希望的，因为PACD显示这些细胞系的IC50值为12.5µg/ml6.25µg/ml25µg/ml和100µg/ml。这项研究强调了PACD作为一种工具的多功能性和潜力，在传感应用和肿瘤学研究中。
