PDF(Pubmed)
Abstract：
Precision Cut Lung Slices (PCLS) have emerged as a sophisticated and physiologically relevant ex vivo model for studying the intricacies of lung diseases, including fibrosis, injury, repair, and host defense mechanisms. This innovative methodology presents a unique opportunity to bridge the gap between traditional in vitro cell cultures and in vivo animal models, offering researchers a more accurate representation of the intricate microenvironment of the lung. PCLS require the precise sectioning of lung tissue to maintain its structural and functional integrity. These thin slices serve as invaluable tools for various research endeavors, particularly in the realm of airway diseases. By providing a controlled microenvironment, precision-cut lung slices empower researchers to dissect and comprehend the multifaceted interactions and responses within lung tissue, thereby advancing our understanding of pulmonary pathophysiology.
摘要：
精确切肺切片（PCLS）已成为一种复杂且生理相关的离体模型，用于研究肺部疾病的复杂性。包括纤维化,损伤,修复,和主机防御机制。这种创新的方法为弥合传统的体外细胞培养和体内动物模型之间的差距提供了独特的机会。为研究人员提供了一个更准确的肺复杂微环境的表示。PCLS需要肺组织的精确切片以保持其结构和功能完整性。这些薄片是各种研究工作的宝贵工具，特别是在气道疾病领域。通过提供受控的微环境，精确切割的肺切片使研究人员能够解剖和理解肺组织内多方面的相互作用和反应，从而提高我们对肺部病理生理学的理解。
