Abstract：
Wheat straw contains a high amount of lignin, hindering the action of cellulase and hemicellulase enzymes, leading to difficulties in nutrient absorption by animals from straw feed. However, currently, the biological treatment of straw relies primarily on fungal degradation and cannot be directly utilized for the preparation of livestock feed. This study focuses on enzymatic co-fermentation of wheat straw to produce high-protein, low-cellulose biological feed, integrating lignin degradation with feed manufacturing, thereby simplifying the feed production process. After the optimization using Box-Behnken Design for the feed formulation, with a glucose oxidase addition of 2.46%, laccase addition of 3.4%, and malonic acid addition of 0.6%, the wheat straw feed prepared in this experiment exhibited a true protein content of 9.35%. This represented a fourfold increase compared to the non-fermented state, and the lignocellulose degradation rate of wheat straw reached 45.42%. These results not only highlight the substantial enhancement in protein content but also underscore the significant advancement in lignocellulose breakdown. This formulation significantly enhanced the palatability and nutritional value of the straw feed, contributing to the industrial development of straw feed.
摘要：
小麦秸秆含有大量的木质素，阻碍纤维素酶和半纤维素酶的作用，导致动物从秸秆饲料中吸收营养的困难。然而,目前,秸秆的生物处理主要依靠真菌降解，不能直接用于制备家畜饲料。本研究的重点是小麦秸秆的酶共发酵生产高蛋白，低纤维素生物饲料,将木质素降解与饲料生产相结合，从而简化了饲料生产过程。在使用Box-BehnkenDesign对饲料配方进行优化后，添加2.46%的葡萄糖氧化酶，漆酶添加量为3.4%，和丙二酸添加0.6%，本实验制备的麦草饲料的真实蛋白质含量为9.35%。与非发酵状态相比，这增加了四倍，小麦秸秆的木质纤维素降解率达到45.42%。这些结果不仅突出了蛋白质含量的实质性增加，而且强调了木质纤维素分解的显着进步。这种配方显著提高了秸秆饲料的适口性和营养价值,促进秸秆饲料的产业化发展。
