Abstract：
Etomidate (ET), a hypnotic agent used for the induction of anesthesia, is rapidly metabolized to etomidate acid (ETA) in the liver. Recently, ET has become one of the most serious alternative drugs of abuse in China. Therefore, an urgent need exists to develop a fast and convenient analysis method for monitoring ET. The current work presents a simple, fast, and sensitive direct injection method for the determination of ET and ETA in wastewater. After the optimization of the ultra-performance liquid chromatography-tandem mass spectrometry and sample filtration conditions, the method exhibited satisfactory limits of detection (1 ng/L) and good filtration loss. The validated method was successfully applied to determine the concentrations of ET and ETA in wastewater samples (n = 245) from several wastewater treatment plants in China. The concentrations of the targets in positive samples ranged from less than the lower limits of quantitation to 47.71 ng/L. The method can meet ET monitoring and high-throughput analysis requirements.
摘要：
依托咪酯(ET),一种用于麻醉诱导的催眠药，在肝脏中迅速代谢为依托咪酯酸（ETA）。最近,ET已成为中国滥用最严重的替代药物之一。因此,迫切需要开发一种快速方便的监测ET的分析方法。目前的工作提出了一个简单的，快,和灵敏的直接进样法测定废水中ET和ETA。经过超高效液相色谱-串联质谱和样品过滤条件的优化,该方法具有令人满意的检测限（1ng/L）和良好的过滤损失。经过验证的方法已成功用于测定中国几个污水处理厂废水样品（n=245）中ET和ETA的浓度。阳性样品中靶标的浓度范围从低于定量下限到47.71ng/L。该方法可以满足ET监测和高通量分析的要求。
