PDF(Pubmed)
Abstract：
Recent studies have shown that blue light-emitting diode (LED) light has anti-tumor effects, suggesting the possibility of using visible light in cancer therapy. However, the effects of blue light irradiation on cells in the tumor microenvironment, including tumor-associated macrophages (TAMs), are unknown. Here, THP-1 cells were cultured in the conditioned medium (CM) of HCT-116 cells to prepare TAMs. TAMs were divided into LED-irradiated and control groups. Then, the effects of blue LED irradiation on TAM activation were examined. Expression levels of M2 macrophage markers CD163 and CD206 expression were significantly decreased in LED-irradiated TAMs compared with the control group. While control TAM-CM could induce HCT-116 cell migration, these effects were not observed in cells cultured in TAM-CM with LED irradiation. Vascular endothelial growth factor (VEGF) secretion was significantly suppressed in LED-exposed TAMs. PD-L1 expression was upregulated in HCT-116 cells cultured with TAM-CM but attenuated in cells cultured with LED-irradiated TAM-CM. In an in vivo model, protein expression levels of F4/80 and CD163, which are TAM markers, were reduced in the LED-exposed group. These results indicate that blue LED light may have an inhibitory effect on TAMs, as well as anti-tumor effects on colon cancer cells.
摘要：
最近的研究表明，蓝色发光二极管（LED）灯具有抗肿瘤作用，表明在癌症治疗中使用可见光的可能性。然而,蓝光照射对肿瘤微环境中细胞的影响，包括肿瘤相关巨噬细胞（TAMs），是未知的。这里,将THP-1细胞在HCT-116细胞的条件培养基（CM）中培养以制备TAM。将TAM分为LED照射组和对照组。然后，研究了蓝光LED照射对TAM激活的影响。与对照组相比，LED照射的TAM中M2巨噬细胞标志物CD163和CD206的表达水平显着降低。而对照TAM-CM可以诱导HCT-116细胞迁移，在LED照射的TAM-CM中培养的细胞中未观察到这些作用。在LED照射的TAM中，血管内皮生长因子（VEGF）的分泌受到显着抑制。在TAM-CM培养的HCT-116细胞中，PD-L1的表达上调，但在LED照射的TAM-CM培养的细胞中，PD-L1的表达减弱。在体内模型中,F4/80和CD163的蛋白表达水平，它们是TAM标记，在LED暴露组中减少。这些结果表明，蓝色LED光可能对TAM具有抑制作用，以及对结肠癌细胞的抗肿瘤作用。
