PDF(Pubmed)
Abstract：
The citral-type is the most common chemotype in Cinnamomum bodinieri Levl (C. bodinieri), which has been widely used in the daily necessities, cosmetics, biomedicine, and aromatic areas due to their high citral content. Despite of this economic prospect, the possible gene-regulatory roles of citral biosynthesis in the same geographic environment remains unknown. In this study, the essential oils (EOs) of three citral type (B1, B2, B3) and one non-citral type (B0) varieties of C. bodinieri were identified by GC-MS after hydrodistillation extraction in July. 43 components more than 0.10% were identified in the EOs, mainly composed of monoterpenes (75.8-91.84%), and high content citral (80.63-86.33%) were identified in citral-type. Combined transcriptome and metabolite profiling analysis, plant-pathogen interaction(ko04626), MAPK signaling pathway-plant(ko04016), starch and sucrose metabolism(ko00500), plant hormone signal transduction(ko04075), terpenoid backbone biosynthesis (ko00900) and monoterpenoid biosynthesis (ko00902) pathways were enriched significantly. The gene expression of differential genes were linked to the monoterpene content, and the geraniol synthase (CbGES), alcohol dehydrogenase (CbADH), geraniol 8-hydroxylase-like (CbCYP76B6-like) and 8-hydroxygeraniol dehydrogenase (Cb10HGO) were upregulated in the citral-type, indicating that they were associated with high content of geraniol and citral. The activities of CbGES and CbADH in citral type were higher than in non-citral type, which was corroborated by enzyme-linked immunosorbent assay (ELISA). This study on the accumulation mechanism of citral provides a theoretical basis for the development of essential oil of C. bodinieri.
摘要：
柠檬醛型是CinnamomumbodinieriLevl中最常见的化学型（C.bodinieri),已被广泛用于日用品中，化妆品,生物医学,和芳香区由于其高柠檬醛含量。尽管有这样的经济前景，柠檬醛生物合成在同一地理环境中可能的基因调控作用仍然未知。在这项研究中,7月，在加氢蒸馏提取后，通过GC-MS鉴定了三种柠檬醛型（B1，B2，B3）和一种非柠檬醛型（B0）品种的精油（EO）。在EO中确定了超过0.10%的43个组件，主要由单萜(75.8-91.84%)组成，在柠檬醛型中发现了高含量的柠檬醛（80.63-86.33％）。结合转录组和代谢物谱分析，植物-病原体相互作用（ko04626），MAPK信号通路-植物(ko04016),淀粉和蔗糖代谢（ko00500），植物激素信号转导（ko04075），萜类化合物骨架生物合成(ko00900)和单萜化合物生物合成(ko00902)途径显著富集。差异基因的基因表达与单萜含量有关，和香叶醇合成酶（CbGES），乙醇脱氢酶（CbADH），香叶醇8-羟化酶样（CbCYP76B6样）和8-羟基香叶醇脱氢酶（Cb10HGO）在柠檬醛型中上调，表明它们与香叶醇和柠檬醛的高含量有关。柠檬醛型CbGES和CbADH活性高于非柠檬醛型,通过酶联免疫吸附测定（ELISA）证实。本研究对柠檬醛的积累机理进行了研究,为木耳精油的开发提供了理论依据。
