PDF(Pubmed)
Abstract：
The close link between intestinal microbiota and bone health (\'gut-bone\' axis) has recently been revealed: the modulation of the amount and nature of bacteria present in the intestinal tract has an impact on bone health and calcium (Ca) metabolism. Probiotics are known to favorably impact the intestinal microbiota. The objective of this study was to investigate the effect of Pediococcus acidilactici CNCM I-4622 (PA) on laying performance, egg/eggshell quality, Ca metabolism and bone mineralization and resistance in relatively old layers (50 wks old at the beginning of the experiment) during 14 weeks. 480 Hy Line brown layers were divided into 2 groups (CON and PA: 3 layers/rep, 80 rep/group) and fed with a diet formulated to be suboptimal in calcium (Ca) and phosphorus (P) (- 10% of the requirements). The total egg weight was improved by 1.1% overall with PA, related to an improvement of the weight of marketable eggs (+ 0.9%). PA induced a decreased % of downgraded eggs, mainly broken eggs (- 0.4 pts) and FCR improvement (- 0.8% for all eggs, - 0.9% for marketable eggs). PA also led to higher Haugh units (HU: + 7.4%). PA tended to decrease crypt depth after the 14 weeks of supplementation period in the jejunum (- 25.2%) and ileum (- 17.6%). As a consequence, the VH/CD ratio appeared increased by PA at the end of the trial in the jejunum (+ 63.0%) and ileum (+ 48.0%). Ca and P retention were increased by 4 pts following PA supplementation, translating into increased bone hardness (+ 19%), bone cohesiveness (+ 43%) and bone Ca & P (+ 1 pt) for PA-supplemented layers. Blood Ca and P were respectively improved by 5% and 12% with PA. In addition, blood calcitriol and osteocalcin concentrations were respectively improved by + 83% and + 3% in PA group at the end of the trial, compared to CON group. There was no difference between the 2 groups for ALP (alkaline phosphatase) and PTH (parathyroid hormone). PA significantly decreased the expression of the following genes: occludin in the small intestine, calbindin 1 in the ovarian tissue and actin B in the bone. PA therefore improved zootechnical performance of these relatively old layers, and egg quality. The parallel increase in Ca and P in the blood and in the bone following PA supplementation suggests an improvement of the mineral supply for eggshell formation without impacting bone integrity, and even increasing bone resistance.
摘要：
最近发现了肠道微生物群与骨骼健康（“肠-骨”轴）之间的紧密联系：肠道中存在的细菌的数量和性质的调节对骨骼健康和钙（Ca）代谢有影响。已知益生菌有利地影响肠道微生物群。本研究的目的是研究乳酸片球菌CNCMI-4622（PA）对产蛋性能的影响，鸡蛋/蛋壳质量，在14周内，相对较老的层（实验开始时50周龄）中的钙代谢和骨矿化和抵抗力。480Hy线棕色层分为2组（CON和PA：3层/代表，80代表/组），并饲喂钙（Ca）和磷（P）次优的饮食（-10％的需求）。PA使鸡蛋总重量总体提高了1.1％，与可销售鸡蛋重量的改善有关（0.9％）。PA诱导降低了降级鸡蛋的百分比，主要是破碎的鸡蛋（-0.4分）和FCR改善（所有鸡蛋-0.8%，-有市场的鸡蛋为0.9%）。PA还导致更高的Haugh单位（HU：7.4％）。在14周的补充期后，PA倾向于降低空肠（-25.2％）和回肠（-17.6％）的隐窝深度。因此,在试验结束时,空肠(+63.0%)和回肠(+48.0%)VH/CD比值出现PA升高.补充PA后，Ca和P保留增加了4个百分点，转化为增加的骨硬度（+19%），补充PA的层的骨粘结性（43％）和骨Ca＆P（1pt）。血Ca和P分别提高5%和12%。此外,在试验结束时，PA组的血骨化三醇和骨钙蛋白浓度分别提高了+83%和+3%，与CON组相比。ALP(碱性磷酸酶)和PTH(甲状旁腺激素)两组间无差异。PA显著降低了以下基因的表达：小肠中的occludin，钙结合蛋白1在卵巢组织中，肌动蛋白B在骨骼中。因此，PA改善了这些相对较旧的层的动物园技术性能，鸡蛋质量。补充PA后，血液和骨骼中Ca和P的平行增加表明蛋壳形成的矿物质供应改善，而不会影响骨骼完整性。甚至增加骨骼阻力。
