Abstract：
OBJECTIVE: Thin endometrium (TE) compromises endometrial receptivity, often leading to implantation failure and lower clinical pregnancy rates. As autologous platelet-rich plasma (PRP) emerges as a potential remedy, the present study focused on its therapeutic effects on TE in infertile women who underwent frozen embryo transfer.
METHODS: Patients with TE who underwent frozen embryo transfer treatment in our hospital were included. To diminish individual variability, a self-controlled series approach was used. Two menstrual study cycles were arranged for each participant before the actual embryo transfer cycle; PRP treatment was conducted in the second cycle. Key metrics analyzed included endometrial thickness and the expression of specific endometrial biomarkers including HOXA-10, Ki67, and αvβ3 integrin. Transvaginal ultrasound was employed to measure endometrial thickness on Days 11 and 14, and an endometrial biopsy was conducted on progesterone Day 5 of the first two cycles. Pregnancy outcomes were observed after the embryo transfer cycle.
RESULTS: PRP treatment significantly increased the median endometrial thickness, from 5.8 mm to 6.5 mm (P = 0.0066). Additionally, PRP treatment resulted in a statistically significant increase in the H-score for all endometrial markers. Importantly, during the subsequent embryo transfer cycle with PRP treatment, two patients successfully achieved pregnancies, both culminating in live births.
CONCLUSIONS: These findings emphasize the potential of PRP in improving endometrial conditions, especially for individuals grappling with thin endometrium issues, as underscored by this self-comparison methodology.
METHODS: Patients with TE who underwent frozen embryo transfer treatment in our hospital were included. To diminish individual variability, a self-controlled series approach was used. Two menstrual study cycles were arranged for each participant before the actual embryo transfer cycle; PRP treatment was conducted in the second cycle. Key metrics analyzed included endometrial thickness and the expression of specific endometrial biomarkers including HOXA-10, Ki67, and αvβ3 integrin. Transvaginal ultrasound was employed to measure endometrial thickness on Days 11 and 14, and an endometrial biopsy was conducted on progesterone Day 5 of the first two cycles. Pregnancy outcomes were observed after the embryo transfer cycle.
RESULTS: PRP treatment significantly increased the median endometrial thickness, from 5.8 mm to 6.5 mm (P = 0.0066). Additionally, PRP treatment resulted in a statistically significant increase in the H-score for all endometrial markers. Importantly, during the subsequent embryo transfer cycle with PRP treatment, two patients successfully achieved pregnancies, both culminating in live births.
CONCLUSIONS: These findings emphasize the potential of PRP in improving endometrial conditions, especially for individuals grappling with thin endometrium issues, as underscored by this self-comparison methodology.
摘要：
目的：薄型子宫内膜（TE）损害子宫内膜容受性，通常导致植入失败和临床妊娠率降低。随着自体富血小板血浆（PRP）成为一种潜在的治疗方法，本研究的重点是其对接受冷冻胚胎移植的不育妇女的治疗效果。
方法：纳入我院接受冷冻胚胎移植治疗的TE患者。为了减少个体差异，采用自控系列方法。在实际胚胎移植周期之前为每个参与者安排两个月经周期；在第二个周期进行PRP治疗。分析的关键指标包括子宫内膜厚度和特定子宫内膜生物标志物(包括HOXA-10、Ki67和αvβ3整联蛋白)的表达。在第11天和第14天采用经阴道超声测量子宫内膜厚度,并且在前两个周期的第5天进行子宫内膜活检。观察胚胎移植周期后的妊娠结局。
结果：PRP治疗显著增加子宫内膜厚度中位数，从5.8毫米到6.5毫米（P=0.0066）。此外,PRP治疗导致所有子宫内膜标记物的H评分在统计学上显著增加。重要的是,在随后的PRP治疗胚胎移植周期中，两名患者成功怀孕，两者都以活产告终。
结论：这些发现强调了PRP改善子宫内膜状况的潜力，特别是对于患有子宫内膜薄问题的个体，正如这种自我比较方法所强调的那样。
方法：纳入我院接受冷冻胚胎移植治疗的TE患者。为了减少个体差异，采用自控系列方法。在实际胚胎移植周期之前为每个参与者安排两个月经周期；在第二个周期进行PRP治疗。分析的关键指标包括子宫内膜厚度和特定子宫内膜生物标志物(包括HOXA-10、Ki67和αvβ3整联蛋白)的表达。在第11天和第14天采用经阴道超声测量子宫内膜厚度,并且在前两个周期的第5天进行子宫内膜活检。观察胚胎移植周期后的妊娠结局。
结果：PRP治疗显著增加子宫内膜厚度中位数，从5.8毫米到6.5毫米（P=0.0066）。此外,PRP治疗导致所有子宫内膜标记物的H评分在统计学上显著增加。重要的是,在随后的PRP治疗胚胎移植周期中，两名患者成功怀孕，两者都以活产告终。
结论：这些发现强调了PRP改善子宫内膜状况的潜力，特别是对于患有子宫内膜薄问题的个体，正如这种自我比较方法所强调的那样。
