PDF(Pubmed)
Abstract：
Campylobacter hepaticus, the causative agent of Spotty Liver Disease (SLD) is an important disease in cage-free egg producing chickens causing mortality and production drops. C. hepaticus is a slow growing Campylobacter easily overgrown by fecal bacteria. It is currently only reliably isolatable from bile samples. A selective media for isolation from feces or environment would assist diagnosis and impact assessment. Growth of five Australian C. hepaticus isolates was studied using Horse blood agar (HBA), sheep blood agar (SBA), Bolton, Preston and Brain Heart Infusion (BHI) base media. Blood and/or bile were added to Bolton, Preston and BHI medias. C. jejuni was used as a positive control. Plates were incubated in duplicate under microaerophilic conditions at 42°C for 10 days and examined at days 3-5 and 7-10 of incubation. Each isolate was examined for sensitivity to 14 antimicrobials using HBA sensitivity plates. Growth was inhibited by BHI and by added bile, while blood improved growth. Further replicates using SBA, HBA, Bolton and Preston media showed best growth on Bolton agar with blood. All five C. hepaticus isolates were resistant to trimethoprim and vancomycin, while four were also resistant to rifampicin and bacitracin. Media based upon Bolton plus blood supplemented with vancomycin and trimethoprim might be used as the most appropriate media for selective growth of C. hepaticus. The addition of bile to media for C. hepaticus isolation and growth will inhibit growth and is not advised.
摘要：
肝弯曲杆菌,该病(SLD)是无笼产蛋鸡的一种重要疾病,可导致死亡率和产量下降。肝肠杆菌是一种生长缓慢的弯曲杆菌，容易被粪便细菌过度生长。它目前只能可靠地从胆汁样品中分离出来。用于从粪便或环境中分离的选择性介质将有助于诊断和影响评估。使用马血琼脂（HBA）研究了五种澳大利亚肝肠杆菌分离株的生长，羊血琼脂（SBA），博尔顿,Preston和脑心输液（BHI）基础培养基。血液和/或胆汁被添加到博尔顿，普雷斯顿和BHI媒体。使用空肠杆菌作为阳性对照。将板在微需氧条件下在42°C下一式两份孵育10天,并在孵育的第3-5天和第7-10天进行检查。使用HBA敏感性板检查每个分离株对14种抗微生物剂的敏感性。BHI和添加的胆汁抑制了生长，而血液改善了生长。使用SBA进一步复制，HBA,Bolton和Preston培养基在具有血液的Bolton琼脂上显示出最佳生长。所有5株肝梭菌分离株对甲氧苄啶和万古霉素均耐药，而四个人也对利福平和杆菌肽耐药。基于Bolton加上补充有万古霉素和甲氧苄啶的血液的培养基可用作选择性生长肝梭菌的最合适的培养基。将胆汁添加到用于分离和生长的肝梭状芽胞杆菌的培养基中将抑制生长并且不建议。
