Abstract：
BACKGROUND: Liqi Tongbian is a traditional Chinese medicine (TCM) preparation that contains herbs that may treat slow transit constipation (STC). Atractylodes macrocephala, Astragalus membranaceus, Fructus aurantii, radish seed, uncooked Polygonum multiflorum, and Agastache rugosa were included in the formula for their unique qualities. The control of water transfer in the colon is greatly influenced by aquaporin 3 (AQP3).
OBJECTIVE: Based on this, the Liqi Tongbian mixture was used to detect the concentrations of aquaporins (AQPs), 5-HT and nitrix oxide synthase 1 (NOS1) in STC rats, and explore its effect, in order to provide a theoretical basis for the remedy of STC with TCM.
METHODS: Zhejiang University of Traditional Chinese Medicine provided 32 three-week-old Sprague Dawley rats of SPF-grade. The pairs licensed under SYXK (Zhejiang) 2021-0012 were kept at 20-25°C and humidity of 50-65%. The compound diphenoxylate caused constipation in the control, model, Liqi laxative (LQTB), and mosapride groups. The Liqi laxative rats were administered a mixture of traditional Chinese herbs after modeling, while mosapride was given to the other group. The levels of 5-HT, NOS1 and AQPs were tested in the feces and intestinal tissues.
RESULTS: Comparing the condition of rat feces, it was found that the model group had significantly lower overall bulk, score and particles within 24 h compared to the control group. In comparison to mosapride, LQTB performed better. The model group had higher levels of 5-HT and NOS1 in intestinal tissue, while the LQTB and mosapride groups had decreased levels of these AQPs. LQTB had lower levels of AQP1, AQP3 and AQP4 than mosapride, while the model group had higher levels of these AQPs.
CONCLUSIONS: Liqi Tongbian mixture works better than mosapride in improving constipation symptoms in rats with STC, and its mechanism is related to regulating the level of intestinal AQPs and neurotransmitters.
OBJECTIVE: Based on this, the Liqi Tongbian mixture was used to detect the concentrations of aquaporins (AQPs), 5-HT and nitrix oxide synthase 1 (NOS1) in STC rats, and explore its effect, in order to provide a theoretical basis for the remedy of STC with TCM.
METHODS: Zhejiang University of Traditional Chinese Medicine provided 32 three-week-old Sprague Dawley rats of SPF-grade. The pairs licensed under SYXK (Zhejiang) 2021-0012 were kept at 20-25°C and humidity of 50-65%. The compound diphenoxylate caused constipation in the control, model, Liqi laxative (LQTB), and mosapride groups. The Liqi laxative rats were administered a mixture of traditional Chinese herbs after modeling, while mosapride was given to the other group. The levels of 5-HT, NOS1 and AQPs were tested in the feces and intestinal tissues.
RESULTS: Comparing the condition of rat feces, it was found that the model group had significantly lower overall bulk, score and particles within 24 h compared to the control group. In comparison to mosapride, LQTB performed better. The model group had higher levels of 5-HT and NOS1 in intestinal tissue, while the LQTB and mosapride groups had decreased levels of these AQPs. LQTB had lower levels of AQP1, AQP3 and AQP4 than mosapride, while the model group had higher levels of these AQPs.
CONCLUSIONS: Liqi Tongbian mixture works better than mosapride in improving constipation symptoms in rats with STC, and its mechanism is related to regulating the level of intestinal AQPs and neurotransmitters.
摘要：
背景：理气通便是一种中药（TCM）制剂，含有可治疗慢传输型便秘（STC）的草药。白术,黄芪，乌兰地,萝卜籽,未煮熟的何首乌，和Agastacherugosa因其独特的品质而被包括在配方中。水通道蛋白3（AQP3）极大地影响了结肠中水转移的控制。
目标：基于此，采用理气通便合剂检测水通道蛋白(AQPs)的浓度,STC大鼠的5-HT和一氧化氮合酶1（NOS1）并探索其效果，以期为中医治疗STC提供理论依据。
方法：浙江中医药大学提供32只SPF级3周龄SD大鼠。根据SYXK（浙江）2021-0012许可的配对保持在20-25°C和50-65％的湿度下。复方苯乙氧基化物在对照组中引起便秘，模型，理气泻药（LQTB），和莫沙必利组。理气泻药大鼠造模后给予中药合剂,而莫沙必利则给予另一组。5-HT的水平，在粪便和肠组织中测试NOS1和AQPs。
结果：比较大鼠粪便的状况，发现模型组的总体体积明显较低，与对照组相比，24小时内的评分和颗粒。与莫沙必利相比，LQTB表现更好。模型组肠组织中5-HT和NOS1水平升高,而LQTB和莫沙必利组的这些AQP水平降低。LQTB的AQP1、AQP3和AQP4水平低于莫沙必利，而模型组的这些AQP水平较高。
结论：理气通便合剂在改善STC大鼠便秘症状方面优于莫沙必利，其机制与调节肠道AQPs和神经递质水平有关。
目标：基于此，采用理气通便合剂检测水通道蛋白(AQPs)的浓度,STC大鼠的5-HT和一氧化氮合酶1（NOS1）并探索其效果，以期为中医治疗STC提供理论依据。
方法：浙江中医药大学提供32只SPF级3周龄SD大鼠。根据SYXK（浙江）2021-0012许可的配对保持在20-25°C和50-65％的湿度下。复方苯乙氧基化物在对照组中引起便秘，模型，理气泻药（LQTB），和莫沙必利组。理气泻药大鼠造模后给予中药合剂,而莫沙必利则给予另一组。5-HT的水平，在粪便和肠组织中测试NOS1和AQPs。
结果：比较大鼠粪便的状况，发现模型组的总体体积明显较低，与对照组相比，24小时内的评分和颗粒。与莫沙必利相比，LQTB表现更好。模型组肠组织中5-HT和NOS1水平升高,而LQTB和莫沙必利组的这些AQP水平降低。LQTB的AQP1、AQP3和AQP4水平低于莫沙必利，而模型组的这些AQP水平较高。
结论：理气通便合剂在改善STC大鼠便秘症状方面优于莫沙必利，其机制与调节肠道AQPs和神经递质水平有关。
