Abstract：
In recent years, rapid advances in research methods have made single cell analysis possible. Systemic sclerosis (SSc), a disease characterized by the triad of immune abnormalities, fibrosis, and vasculopathy, has also been the subject of various analyses. To summarize the results of single cell analysis in SSc accumulated to date and to deepen our understanding of SSc. Four databases were used to perform a database search on 23rd June 2023. Assessed Grading of Recommendations Assessment, Development and Evaluation certainty of evidence were performed according to PRISMA guidelines. The analysis was completed on July 2023. 17 studies with 358 SSc patients were included. Three studies used PBMCs, six used skin, nine used lung with SSc-interstitial lung diseases (ILDs), and one used lung with SSc-pulmonary arterial hypertension (PAH). The cells studied included immune cells such as T cells, natural killer cells, monocytes, macrophages, and dendritic cells, as well as endothelial cells, fibroblasts, keratinocytes, alveolar type I cells, basal epithelial cells, smooth muscle cells, mesothelial cells, etc. This systematic review revealed the results of single cell analysis, suggesting that PBMCs, skin, SSc-ILD, and SSc-PAH show activation and dysfunction of cells associated with immune-abnormalities, fibrosis, and vasculopathy, respectively.
摘要：
近年来,研究方法的快速发展使单细胞分析成为可能。系统性硬化症（SSc），一种以免疫异常为特征的疾病，纤维化，血管病变,也是各种分析的主题。总结迄今为止积累的SSc单细胞分析结果，加深对SSc的理解。2023年6月23日，使用了四个数据库进行数据库搜索。建议评估的评估等级，根据PRISMA指南制定和评估证据的确定性。该分析于2023年7月完成。纳入了358名SSc患者的17项研究。三项研究使用PBMC，六种用过的皮肤，9例患有SSc间质性肺病（ILD）的二手肺，和一个使用肺SSc肺动脉高压（PAH）。研究的细胞包括免疫细胞，如T细胞，自然杀伤细胞,单核细胞,巨噬细胞，和树突状细胞，以及内皮细胞，成纤维细胞,角质形成细胞，肺泡I型细胞，基底上皮细胞，平滑肌细胞，间皮细胞,等。本系统综述揭示了单细胞分析的结果，表明PBMC，皮肤,SSc-ILD,和SSc-PAH显示与免疫异常相关的细胞的激活和功能障碍，纤维化，血管病变,分别。
