PDF(Pubmed)
Abstract：
This study investigated the effects of pregabalin on microglial differentiation in rats with neuropathic pain (NP) induced by sciatic nerve ligation and transection. After confirming NP, the rats were randomly allocated to either a pregabalin or control group. The pregabalin group received intraperitoneal injections of 10 mg/kg pregabalin, while the control group received an equivalent volume of normal saline following surgery. On postoperative day 28, neuronal damage, microglial activity, and microglial differentiation were assessed. The pregabalin group exhibited significantly less neuronal damage compared to the control group, along with a significant decrease in activated microglial expression in both the brain and spinal cord. Pregabalin treatment also significantly altered the microglial phenotype expression, with a decrease in the M1 phenotype percentage and an increase in the M2 phenotype percentage in both the brain (M1 phenotype: 43.52 ± 12.16% and 18.00 ± 8.57% in the control and pregabalin groups, respectively; difference: 27.26 [15.18-42.10], p = 0.002; M2 phenotype: 16.88 ± 6.47% and 39.63 ± 5.82% in the control and pregabalin groups, respectively; difference 22.04 [17.17-32.70], p < 0.001) and the spinal cord ipsilateral to nerve injury (M1 phenotype: 44.35 ± 12.12% and 13.78 ± 5.39% in the control and pregabalin groups, respectively; difference 30.46 [21.73-44.45], p < 0.001; M2 phenotype: 7.64 ± 3.91% and 33.66 ± 7.95% in the control and pregabalin groups, respectively; difference 27.41 [21.21-36.30], p < 0.001). Overall, pregabalin treatment significantly decreased the microglial M1 phenotype while increasing the microglial M2 phenotype in NP rats.
摘要：
本研究探讨了普瑞巴林对坐骨神经结扎横断所致神经性疼痛(NP)大鼠小胶质细胞分化的影响。确认NP后，将大鼠随机分为普瑞巴林组和对照组.普瑞巴林组腹腔注射10mg/kg普瑞巴林,而对照组在手术后接受等量的生理盐水。术后第28天，神经元损伤，小胶质细胞活动，和小胶质细胞分化进行评估。与对照组相比，普瑞巴林组表现出明显更少的神经元损伤，伴随着大脑和脊髓中激活的小胶质细胞表达的显着降低。普瑞巴林治疗也显著改变了小胶质细胞表型表达，随着M1表型百分比的降低和M2表型百分比的增加，在两个大脑中（M1表型：在对照组和普瑞巴林组中为43.52±12.16％和18.00±8.57％，分别；差异：27.26[15.18-42.10]，p=0.002;M2表型:对照组和普瑞巴林组分别为16.88±6.47%和39.63±5.82%,分别；差异22.04[17.17-32.70]，p<0.001）和脊髓同侧神经损伤（M1表型：对照组和普瑞巴林组的44.35±12.12％和13.78±5.39％，分别；差异30.46[21.73-44.45]，p<0.001;M2表型:对照组和普瑞巴林组分别为7.64±3.91%和33.66±7.95%,分别；差异27.41[21.21-36.30]，p<0.001)。总的来说,普瑞巴林治疗显著降低NP大鼠小胶质细胞M1表型,同时增加小胶质细胞M2表型。
