PDF(Pubmed)
Abstract：
Traumatic brain injury (TBI) is a leading cause of morbidity, disability, and mortality worldwide. Motor and cognitive deficits and emotional disturbances are long-term consequences of TBI. A lack of effective treatment for TBI-induced neural damage, functional impairments, and cognitive deficits makes it challenging in the recovery following TBI. One of the reasons may be the lack of knowledge underlying the complex pathophysiology of TBI and the regulatory factors involved in the cellular and molecular mechanisms of inflammation, neural regeneration, and injury repair. These mechanisms involve a change in the expression of various proteins encoded by genes whose expression is regulated by transcription factors (TFs) at the transcriptional level and microRNA (miRs) at the mRNA level. In this pilot study, we performed the RNA sequencing of injured tissues and non-injured tissues from the brain of Yucatan miniswine and analyzed the sequencing data for differentially expressed genes (DEGs) and the TFs and miRs regulating the expression of DEGs using in-silico analysis. We also compared the effect of the electromagnetic field (EMF) applied to the injured miniswine on the expression profile of various DEGs. The results of this pilot study revealed a few DEGs that were significantly upregulated in the injured brain tissue and the EMF stimulation showed effect on their expression profile.
摘要：
创伤性脑损伤（TBI）是发病的主要原因，残疾,和全世界的死亡率。运动和认知缺陷以及情绪障碍是TBI的长期后果。缺乏有效的治疗TBI引起的神经损伤，功能障碍，认知缺陷使得TBI后的恢复具有挑战性。原因之一可能是缺乏关于TBI的复杂病理生理学和参与炎症的细胞和分子机制的调节因素的知识。神经再生,和损伤修复。这些机制涉及由基因编码的各种蛋白质表达的变化，这些基因的表达在转录水平上受到转录因子（TF）和在mRNA水平上受到微小RNA（miR）的调节。在这项试点研究中，我们对尤卡坦小型猪大脑的损伤组织和非损伤组织进行了RNA测序,并使用计算机分析分析了差异表达基因(DEG)和调节DEG表达的TFs和miR的测序数据.我们还比较了电磁场（EMF）对受伤的小酒对各种DEG表达谱的影响。该初步研究的结果揭示了在损伤的脑组织中显著上调的一些DEGs,并且EMF刺激显示出对它们的表达谱的影响。
