Comparison of LIRADS 5 Image Guided Core Biopsy Derived From Formalin Fixed and Frozen Tissue Cores for Radiogenomics and Radioproteomics Analysis in Well, Moderate and Poorly Differentiated Hepatocellular Carcinoma.

比较从福尔马林固定和冷冻组织核衍生的 LIRADS 5 图像引导的核心活检 ， 用于放射基因组学和放射蛋白质组学分析 ，中度和低分化肝细胞癌。

• 发表时间: 2024
• 来源期刊: Glob Med Ther
• PMID: 38818084
• 文章类型: Journal Article
• 作者列表: Simonian M,Lu DSK,Whitelegge J,Cohn W,Ahuja P,Hsu W,Raman SS

关键词: Liver HCC; biomarkers; radiogenomics; radioproteomics

Abstract

The aim of this pilot study is to evaluate and compare the quality of the genomics and proteomics data obtained from paired Formalin Fixed Paraffin Embedded (FFPE) and frozen (FF) tissue percutaneous core biopsies of Liver Imaging Reporting and Data System 5 (LIRADS 5) hepatocellular carcinoma (HCC) of varying histological grades. The preliminary data identified differentially expressed proteins and genes in poor, moderate and well differentiated HCC biopsies, with a greater efficacy in fresh frozen samples. The data offered valuable insights into the characteristics and suitability of samples for future studies.

摘要

这项初步研究的目的是评估和比较从肝脏成像报告和数据系统5（LIRADS5）的配对福尔马林固定石蜡包埋（FFPE）和冷冻（FF）组织经皮核心活检获得的基因组学和蛋白质组学数据的质量。初步数据确定了差异表达的蛋白质和基因，中度和高分化HCC活检，在新鲜冷冻样品中具有更大的功效。这些数据为未来研究提供了对样品特征和适用性的宝贵见解。