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Abstract：
BACKGROUND: Breast and cervical cancer are the two leading cancers in terms of incidence and mortality. Previous studies reported different interleukins, including interleukin-17A (IL-17A) to be responsible for the development and progression of these malignancies. Therefore, we speculated that the variants in this gene might be associated with these cancer developments in Bangladeshi population. For evaluating the hypothesis, we investigated the association of IL-17A rs3748067 polymorphism with the susceptibility of both breast and cervical cancer.
METHODS: This case-control study was performed on 156 breast cancer patients, 156 cervical cancer patients, and 156 controls using the tetra-primer amplification refractory mutation system-polymerase chain reaction. The statistical software package SPSS (version 25.0) was applied for analyses. The genetic association was measured by the odds ratio (OR) and 95% confidence intervals (CIs). A statistically significant association was considered when p-value ≤ 0.05. Functional analysis was performed using GEPIA and UALCAN databases.
RESULTS: From the calculation of the association of IL-17A rs3748067 with breast cancer, it is found that no genotype or allele showed a statistically significant association (p>0.05). On the other hand, the analysis of IL-17A rs3748067 with cervical cancer demonstrated that CT genotype showed a significant association (CT vs. CC: OR=1.79, p=0.021). In the overdominant model, CT genotype also revealed a statistically significant association with cervical cancer, which is found to be statistically significant (OR=1.84, p=0.015).
CONCLUSIONS: Our study summarizes that rs3748067 polymorphism in the IL-17A gene may be associated with cervical cancer but not breast cancer in Bangladeshi patients. However, we suggest studies in the future with a larger sample size.
METHODS: This case-control study was performed on 156 breast cancer patients, 156 cervical cancer patients, and 156 controls using the tetra-primer amplification refractory mutation system-polymerase chain reaction. The statistical software package SPSS (version 25.0) was applied for analyses. The genetic association was measured by the odds ratio (OR) and 95% confidence intervals (CIs). A statistically significant association was considered when p-value ≤ 0.05. Functional analysis was performed using GEPIA and UALCAN databases.
RESULTS: From the calculation of the association of IL-17A rs3748067 with breast cancer, it is found that no genotype or allele showed a statistically significant association (p>0.05). On the other hand, the analysis of IL-17A rs3748067 with cervical cancer demonstrated that CT genotype showed a significant association (CT vs. CC: OR=1.79, p=0.021). In the overdominant model, CT genotype also revealed a statistically significant association with cervical cancer, which is found to be statistically significant (OR=1.84, p=0.015).
CONCLUSIONS: Our study summarizes that rs3748067 polymorphism in the IL-17A gene may be associated with cervical cancer but not breast cancer in Bangladeshi patients. However, we suggest studies in the future with a larger sample size.
摘要：
背景：就发病率和死亡率而言，乳腺癌和宫颈癌是两种主要的癌症。以前的研究报道了不同的白细胞介素，包括白细胞介素-17A（IL-17A）负责这些恶性肿瘤的发展和进展。因此,我们推测该基因的变异可能与孟加拉国人群的这些癌症发展有关.为了评估假设，我们研究了IL-17Ars3748067多态性与乳腺癌和宫颈癌易感性的关系.
方法：这项病例对照研究是对156例乳腺癌患者进行的，156例宫颈癌患者，和156个对照使用四引物扩增难治性突变系统-聚合酶链反应。应用统计软件包SPSS（25.0版）进行分析。通过比值比（OR）和95％置信区间（CI）测量遗传关联。当p值≤0.05时，认为有统计学意义的关联。使用GEPIA和UALCAN数据库进行功能分析。
结果：通过计算IL-17Ars3748067与乳腺癌的相关性，发现没有基因型或等位基因显示有统计学意义的相关性（p>0.05）。另一方面,IL-17Ars3748067与宫颈癌的分析表明，CT基因型显示出显着关联（CT与CC:OR=1.79,p=0.021)。在占主导地位的模型中，CT基因型也揭示了与宫颈癌的统计学显著关联，发现具有统计学意义(OR=1.84,p=0.015)。
结论：我们的研究总结了IL-17A基因的rs3748067多态性可能与孟加拉国患者的宫颈癌有关，但与乳腺癌无关。然而,我们建议将来进行更大样本量的研究。
方法：这项病例对照研究是对156例乳腺癌患者进行的，156例宫颈癌患者，和156个对照使用四引物扩增难治性突变系统-聚合酶链反应。应用统计软件包SPSS（25.0版）进行分析。通过比值比（OR）和95％置信区间（CI）测量遗传关联。当p值≤0.05时，认为有统计学意义的关联。使用GEPIA和UALCAN数据库进行功能分析。
结果：通过计算IL-17Ars3748067与乳腺癌的相关性，发现没有基因型或等位基因显示有统计学意义的相关性（p>0.05）。另一方面,IL-17Ars3748067与宫颈癌的分析表明，CT基因型显示出显着关联（CT与CC:OR=1.79,p=0.021)。在占主导地位的模型中，CT基因型也揭示了与宫颈癌的统计学显著关联，发现具有统计学意义(OR=1.84,p=0.015)。
结论：我们的研究总结了IL-17A基因的rs3748067多态性可能与孟加拉国患者的宫颈癌有关，但与乳腺癌无关。然而,我们建议将来进行更大样本量的研究。
