Abstract：
BACKGROUND: Breast lipofilling, a popular cosmetic and reconstructive procedure, involves the transplantation of autologous fat to enhance breast volume and contour. Despite its widespread use, cell processing and the aftertreatment remain controversial. This study investigates the pressure applied by a compression bra and reports in vitro stress tests of processed and unprocessed fat cells.
METHODS: Clinical bra pressure measurements were conducted on a cohort of 45 patients following lipofilling, reduction mammoplasties and DIEP flaps. Laboratory analysis included cell vitality testing using Resazurin assays of processed and unprocessed fat cells after exposure to mechanical or hyperbaric pressure.
RESULTS: Our findings show a mean overall pressure value of the compression bra for all patients of 6.7 ± 5.7 mmHg (range 0-35). Cell processing is superior to sedimentation only regarding fat cell vitality. However, neither mechanical pressure within the specified range nor hyperbaric oxygen exposure significantly affected fat graft survival as measured by Resazurin assays.
CONCLUSIONS: The in vitro measurements showed that it was impossible to harm fat cells with external pressure during lipofilling procedures, regardless of their processing. In the clinical context, the compression bra applied pressure values deceeding the perfusion pressure and may therefore not diminish oxygen supply nor harm the transplanted cells. Therefore, we recommend the use of a compression bra for all lipofilling procedures around the breast.
METHODS: This journal requires that authors assign a level of evidence to each article. For a full description of these evidence-based medicine ratings, please refer to Table of Contents or online Instructions to Authors www.springer.com/00266.
METHODS: Clinical bra pressure measurements were conducted on a cohort of 45 patients following lipofilling, reduction mammoplasties and DIEP flaps. Laboratory analysis included cell vitality testing using Resazurin assays of processed and unprocessed fat cells after exposure to mechanical or hyperbaric pressure.
RESULTS: Our findings show a mean overall pressure value of the compression bra for all patients of 6.7 ± 5.7 mmHg (range 0-35). Cell processing is superior to sedimentation only regarding fat cell vitality. However, neither mechanical pressure within the specified range nor hyperbaric oxygen exposure significantly affected fat graft survival as measured by Resazurin assays.
CONCLUSIONS: The in vitro measurements showed that it was impossible to harm fat cells with external pressure during lipofilling procedures, regardless of their processing. In the clinical context, the compression bra applied pressure values deceeding the perfusion pressure and may therefore not diminish oxygen supply nor harm the transplanted cells. Therefore, we recommend the use of a compression bra for all lipofilling procedures around the breast.
METHODS: This journal requires that authors assign a level of evidence to each article. For a full description of these evidence-based medicine ratings, please refer to Table of Contents or online Instructions to Authors www.springer.com/00266.
摘要：
背景：乳房脂肪填充，一种流行的美容和重建程序，涉及自体脂肪的移植，以增加乳房的体积和轮廓。尽管它广泛使用，细胞处理和后处理仍然存在争议。这项研究调查了压缩胸罩施加的压力，并报告了加工和未加工脂肪细胞的体外压力测试。
方法：对45名患者进行了临床胸罩压力测量，减少乳房成形术和DIEP皮瓣。实验室分析包括在暴露于机械或高压压力后，使用经过处理和未经处理的脂肪细胞的Resazurin测定法进行细胞活力测试。
结果：我们的发现显示所有患者的压缩胸罩的平均总压力值为6.7±5.7mmHg（范围0-35）。仅就脂肪细胞活力而言，细胞处理优于沉降。然而,指定范围内的机械压力或高压氧暴露均不会显着影响通过瑞舒林测定法测得的脂肪移植物的存活率。
结论：体外测量表明，在脂肪填充过程中，不可能用外部压力伤害脂肪细胞，不管他们的处理。在临床上，压缩胸罩施加的压力值降低灌注压力，因此可能不会减少氧气供应也不会伤害移植细胞。因此,我们建议在乳房周围的所有脂肪填充程序中使用压缩胸罩。
方法：本期刊要求作者为每篇文章分配一定程度的证据。为了完整描述这些循证医学评级，请参阅目录或在线作者说明www。springer.com/00266.
方法：对45名患者进行了临床胸罩压力测量，减少乳房成形术和DIEP皮瓣。实验室分析包括在暴露于机械或高压压力后，使用经过处理和未经处理的脂肪细胞的Resazurin测定法进行细胞活力测试。
结果：我们的发现显示所有患者的压缩胸罩的平均总压力值为6.7±5.7mmHg（范围0-35）。仅就脂肪细胞活力而言，细胞处理优于沉降。然而,指定范围内的机械压力或高压氧暴露均不会显着影响通过瑞舒林测定法测得的脂肪移植物的存活率。
结论：体外测量表明，在脂肪填充过程中，不可能用外部压力伤害脂肪细胞，不管他们的处理。在临床上，压缩胸罩施加的压力值降低灌注压力，因此可能不会减少氧气供应也不会伤害移植细胞。因此,我们建议在乳房周围的所有脂肪填充程序中使用压缩胸罩。
方法：本期刊要求作者为每篇文章分配一定程度的证据。为了完整描述这些循证医学评级，请参阅目录或在线作者说明www。springer.com/00266.
