Abstract：
Collagen sponge and epidermal growth factor (EGF) promote wound healing. However, the effect of collagen sponge combined with EGF in repairing maxillofacial head and neck wounds remains unclear. The rats were divided into 3 groups, including experimental group 1 (Vaseline gauze+EGF), experimental group 2 (collagen sponge+EGF) with control group (Vaseline+normal saline), and maxillofacial head and neck wounds were simulated. Wound pathological morphology was detected by HE staining; wound EGF, IL-1β, IL-6 along with TNF-α contents by ELISA and MMP1 level by western blot. At 7 and 14 days after treatment, wound healing rate of two experimental groups was higher than that of control group, and that of experimental group 2 presented higher than that of experimental group 1. Compared with control group, experimental group 1 had significantly fewer inflammatory cells in the wound tissue, local erythrocyte spillage outside the vascular walls, more collagen deposition and more granulation tissue. Compared with experimental group 1, inflammatory cells in wound tissues of experimental group 2 were significantly reduced, the collagen tissues were visible and arranged, and the growth of the wound granulation tissue was obvious. IL-1β, IL-6 along with TNF-α levels in two experimental groups presented lower than control group, and EGF level was higher. More importantly, in contrast to experimental group 1, IL-1β, IL-6 along with TNF-α in experimental group 2 presented lower, and EGF level presented higher. At 14 days after treatment, MMP1 level in two experimental groups was lower than control group. In contrast to experimental group 1, MMP1 level in experimental group 2 was lower. In summary, collagen sponge combined with EGF for the first time significantly improved the healing speed of maxillofacial head and neck wounds and reduced the scar left after wound healing.
摘要：
胶原海绵和表皮生长因子(EGF)促进伤口愈合。然而,胶原海绵联合EGF修复颌面部头颈部创面的效果尚不清楚。将大鼠分为3组,包括实验组1（凡士林纱布+EGF），实验组2(胶原海绵+EGF)与对照组(凡士林+生理盐水),模拟颌面部头颈部伤口。HE染色检测创面病理形态学;创面EGF,IL-1β,ELISA检测IL-6与TNF-α含量，Westernblot检测MMP1水平。治疗后第7天和第14天，两组创面愈合率高于对照组,实验组2的表现高于实验组1。与对照组相比,实验组1伤口组织中的炎症细胞明显较少，局部红细胞溢出到血管壁外，更多的胶原沉积和更多的肉芽组织。与实验1组相比,实验2组创面组织中炎性细胞明显减少,胶原组织是可见和排列的，创面肉芽组织生长明显。IL-1β,两个实验组的IL-6和TNF-α水平均低于对照组，EGF水平较高。更重要的是,与实验组1相比，IL-1β，实验组2中IL-6和TNF-α水平较低,EGF水平较高。治疗后14天，两个实验组的MMP1水平均低于对照组。与实验组1相比，实验组2中的MMP1水平较低。总之,胶原海绵联合EGF可显著提高颌面部头颈部创面愈合速度，减少创面愈合后留下的瘢痕。
