PDF(Pubmed)
Abstract：
UNASSIGNED: The salivary microbiome may interact with chemoradiotherapy through dynamic changes in microbial composition and systemic immunity. We aimed to explore the association between the salivary microbiome and response to chemoradiotherapy in initially inoperable patients with local advanced esophageal squamous cell carcinoma (LAESCC).
UNASSIGNED: Salivary and peripheral blood samples were collected before and after chemoradiotherapy. The microbiome and metabolic pathways were analyzed by 16S ribosomal RNA sequencing and liquid chromatography tandem mass spectrometry/Mass spectrometry analyses.
UNASSIGNED: The salivary microbiome exhibited characteristic variations between patients and healthy controls. A significant correlation was found between Prevotella_salivae, Saccharibacteria_TM7_G3_bacterium_HMT_351, and Veillonellaceae_G1_bacterium_HMT_129 and pathological complete response (pCR) in initially inoperable patients who underwent surgery. The PICRUSt suggested that immune diseases and cell motility were different in tumor compared to normal groups. KEGG enrichment analysis showed enriched lipid metabolism, signal transduction, and membrane transport in the tumor group. CD3+CD8 T cells, IL6, IL10, and IFNγ exhibited an increasing trend during the treatment process of chemoradiotherapy.
UNASSIGNED: Our study demonstrated that variations in specific saliva taxa associated with host immunomodulatory cells and cytokines could be promising for early efficacy prediction of chemoradiotherapy in initially inoperable patients with LAESCC.
UNASSIGNED: Salivary and peripheral blood samples were collected before and after chemoradiotherapy. The microbiome and metabolic pathways were analyzed by 16S ribosomal RNA sequencing and liquid chromatography tandem mass spectrometry/Mass spectrometry analyses.
UNASSIGNED: The salivary microbiome exhibited characteristic variations between patients and healthy controls. A significant correlation was found between Prevotella_salivae, Saccharibacteria_TM7_G3_bacterium_HMT_351, and Veillonellaceae_G1_bacterium_HMT_129 and pathological complete response (pCR) in initially inoperable patients who underwent surgery. The PICRUSt suggested that immune diseases and cell motility were different in tumor compared to normal groups. KEGG enrichment analysis showed enriched lipid metabolism, signal transduction, and membrane transport in the tumor group. CD3+CD8 T cells, IL6, IL10, and IFNγ exhibited an increasing trend during the treatment process of chemoradiotherapy.
UNASSIGNED: Our study demonstrated that variations in specific saliva taxa associated with host immunomodulatory cells and cytokines could be promising for early efficacy prediction of chemoradiotherapy in initially inoperable patients with LAESCC.
摘要：
■唾液微生物组可能通过微生物组成和全身免疫力的动态变化与放化疗相互作用。我们的目的是探讨唾液微生物组与最初无法手术的局部晚期食管鳞状细胞癌（LAESCC）患者对放化疗反应之间的关系。
■在放化疗前后收集唾液和外周血样本。通过16S核糖体RNA测序和液相色谱串联质谱/质谱分析来分析微生物组和代谢途径。
■唾液微生物组表现出患者和健康对照之间的特征性差异。发现普雷沃氏菌之间存在显着相关性，最初无法手术的患者接受手术的糖杆菌_TM7_G3_bacterium_HMT_351和Veillonellaceae_G1_bacterium_HMT_129和病理完全反应（pCR）。PICRUSt表明，与正常组相比，肿瘤的免疫性疾病和细胞运动性不同。KEGG富集分析显示丰富的脂质代谢，信号转导，和肿瘤组的膜转运。CD3+CD8T细胞，在放化疗治疗过程中，IL6、IL10和IFNγ呈增加趋势。
■我们的研究表明，与宿主免疫调节细胞和细胞因子相关的特定唾液分类群的变化可能有望在最初无法手术的LAESCC患者中进行放化疗的早期疗效预测。
■在放化疗前后收集唾液和外周血样本。通过16S核糖体RNA测序和液相色谱串联质谱/质谱分析来分析微生物组和代谢途径。
■唾液微生物组表现出患者和健康对照之间的特征性差异。发现普雷沃氏菌之间存在显着相关性，最初无法手术的患者接受手术的糖杆菌_TM7_G3_bacterium_HMT_351和Veillonellaceae_G1_bacterium_HMT_129和病理完全反应（pCR）。PICRUSt表明，与正常组相比，肿瘤的免疫性疾病和细胞运动性不同。KEGG富集分析显示丰富的脂质代谢，信号转导，和肿瘤组的膜转运。CD3+CD8T细胞，在放化疗治疗过程中，IL6、IL10和IFNγ呈增加趋势。
■我们的研究表明，与宿主免疫调节细胞和细胞因子相关的特定唾液分类群的变化可能有望在最初无法手术的LAESCC患者中进行放化疗的早期疗效预测。
