PDF(Pubmed)
Abstract：
The demand for cowhide (ponmo) is currently very high, particularly in Nigeria, due to rising commodity prices, including animal proteins, which has forced a larger percentage of the population who cannot afford meat, chicken, turkey or eggs to rely on other meat products such as \"ponmo,\" \"kundi,\" and \"tinko\" as an alternative source of protein. This research aims to identify microorganisms associated with ponmo, determine the antibiogram of the isolates, and assess the nutritional value of ponmo marketed in Ilishan-Remo central market. Six ponmo vendors were sampled for Dry White Ponmo (DWP), Wet White Ponmo (WWP), Wet Brown Ponmo (WBP) and Brown Ponmo Water (BPW) and transported in sterile containers to the Laboratory for analysis to determine the microbial load, sensitivity, and proximate analysis using standardized methods. For microbiological analysis, samples were tested in triplicate. All samples analyzed had a high microbial load count (from 1.1 x 106 to 1.4 x 107). The organisms isolated were Escherichia coli (34.21 %), Staphylococcus aureus (26.31 %), Klebsiella spp. (18.42 %), Pseudomonas spp. (13.15 %) and Coagulase-negative staphylococci (7.89 %). All the isolates were multidrug-resistant (MDR). Septrin had the highest resistivity (86.84 %) while gentamicin had the lowest resistance (7.89 %). Pefloxacin sensitivity was observed in 37 of the 38 isolates (97.37 % sensitivity). Ciprofloxacin and gentamicin came second and third (84.2 % and 73.68 % sensitivity) respectively. According to the proximate analysis, the WWP has more protein, fat, and fiber, whereas the WBP has more moisture. Food handlers should follow Good Hygiene Practices and take a Food Handlers Test regularly.
摘要：
目前对牛皮（ponmo）的需求非常高，尤其是在尼日利亚，由于商品价格上涨，包括动物蛋白,这迫使更多的人买不起肉，鸡肉,火鸡或鸡蛋依赖其他肉类产品，如“ponmo，\"\"昆迪,\"和\"tinko\"作为蛋白质的替代来源。这项研究旨在鉴定与ponmo相关的微生物，确定分离物的抗菌谱，并评估在Ilishan-Remo中央市场销售的ponmo的营养价值。对六个ponmo供应商进行了DryWhitePonmo（DWP）采样，湿白色Ponmo（WWP），湿布朗庞莫（WBP）和布朗庞莫水（BPW），并在无菌容器中运输到实验室进行分析，以确定微生物负荷，灵敏度,和使用标准化方法进行近似分析。微生物分析，样品一式三份进行测试。所分析的所有样品具有高微生物负荷计数(从1.1X106至1.4X107)。分离出的菌为大肠杆菌(34.21%),金黄色葡萄球菌（26.31%），克雷伯菌属。（18.42%），假单胞菌属。(13.15%)和凝固酶阴性葡萄球菌(7.89%)。所有分离株均为多药耐药（MDR）。Septrin的电阻率最高（86.84％），而庆大霉素的电阻率最低（7.89％）。38株分离株中有37株对培氟沙星敏感(敏感性为97.37%)。环丙沙星和庆大霉素排名第二和第三（敏感性分别为84.2％和73.68％）。根据近似分析，WWP有更多的蛋白质，脂肪，和纤维，而WBP有更多的水分。食品处理人员应遵循良好卫生习惯，并定期进行食品处理人员测试。
