PDF(Pubmed)
Abstract：
UNASSIGNED: Dental caries is a frequently occurring and multifactorial chronic disease in children resulting from the interaction of cariogenic bacteria and host susceptibility. The aim of this study was to elucidate the impacts of primary immunodeficiency disorders (PIDs) on microbiota of dental caries in children by 16S rRNA gene-based metagenomic analysis.
UNASSIGNED: Enrolled in this study were 15 children with primary PID with caries (PID group) and 15 healthy children with caries as a control (CG). The DMFT index, saliva flow rate, and buffering capacity of each participant were assessed before the metagenomic analyses were conducted. For taxonomic profiling, the reads were obtained by high-throughput sequencing of the V3-V4 hypervariable region of 16S rRNA.
UNASSIGNED: The DMFT score, saliva flow rate, and buffering capacity of the groups were similar. The flow rate and buffering capacity had no correlation with the number of species with 95% confidence. The metagenomic analysis resulted in the identification of 2440 bacterial species in all of the samples. Among the 50 most prevalent species present at ≥1% relative abundance, Prevotella melaninogenica and Prevotella salivae were differentially more abundant in the PID group. The PID group and CG showed similar species richness and evenness, but 4 of the 5 samples with the highest Shannon-Weiner and Inverse Simpson indices belonged to the PID group. The Spearman test results for correlation of the species in the PID subgroups showed that Prevotella oris had a positively correlated relationship with both Scardovia wiggsiae and Saccharibacteria genera incertae sedis.
UNASSIGNED: This study provided insight into the caries microbiota of children with immunodeficiency diseases. Differentially abundant species, novel bacterial associations, and unique bacterial species were disclosed in the PID samples, indicating the role of the immune system in altering the caries microbiota. The prominent bacterial species and associations in the PID group should be suspected in regard to their link with present or future diseases.
UNASSIGNED: Enrolled in this study were 15 children with primary PID with caries (PID group) and 15 healthy children with caries as a control (CG). The DMFT index, saliva flow rate, and buffering capacity of each participant were assessed before the metagenomic analyses were conducted. For taxonomic profiling, the reads were obtained by high-throughput sequencing of the V3-V4 hypervariable region of 16S rRNA.
UNASSIGNED: The DMFT score, saliva flow rate, and buffering capacity of the groups were similar. The flow rate and buffering capacity had no correlation with the number of species with 95% confidence. The metagenomic analysis resulted in the identification of 2440 bacterial species in all of the samples. Among the 50 most prevalent species present at ≥1% relative abundance, Prevotella melaninogenica and Prevotella salivae were differentially more abundant in the PID group. The PID group and CG showed similar species richness and evenness, but 4 of the 5 samples with the highest Shannon-Weiner and Inverse Simpson indices belonged to the PID group. The Spearman test results for correlation of the species in the PID subgroups showed that Prevotella oris had a positively correlated relationship with both Scardovia wiggsiae and Saccharibacteria genera incertae sedis.
UNASSIGNED: This study provided insight into the caries microbiota of children with immunodeficiency diseases. Differentially abundant species, novel bacterial associations, and unique bacterial species were disclosed in the PID samples, indicating the role of the immune system in altering the caries microbiota. The prominent bacterial species and associations in the PID group should be suspected in regard to their link with present or future diseases.
摘要：
■龋齿是儿童中由致龋细菌和宿主易感性的相互作用引起的频繁发生和多因素的慢性疾病。这项研究的目的是通过基于16SrRNA基因的宏基因组分析来阐明原发性免疫缺陷疾病（PID）对儿童龋齿微生物群的影响。
■参加本研究的是15名患有龋齿的原发性PID儿童（PID组）和15名患有龋齿的健康儿童作为对照（CG）。DMFT指数，唾液流速,在进行宏基因组分析之前,对每位参与者的缓冲能力进行了评估.对于分类分析，通过16SrRNA的V3-V4高变区的高通量测序获得读数.
■DMFT得分，唾液流速,各组的缓冲能力相似。流速和缓冲能力与物种数量无关，置信度为95％。宏基因组分析导致在所有样品中鉴定出2440种细菌。在相对丰度≥1%的50种最普遍的物种中，PID组中黑色素prevotella和唾液prevotella的含量不同。PID组和CG组显示出相似的物种丰富度和均匀度，但是Shannon-Weiner和InverseSimpson指数最高的5个样本中有4个属于PID组。PID亚组中物种相关性的Spearman检验结果表明，Prevotellaoris与Scardoviawiggziae和accertaesacgrances属均呈正相关。
■这项研究提供了对患有免疫缺陷疾病的儿童龋齿微生物群的见解。差异丰富的物种，新的细菌协会，在PID样本中发现了独特的细菌种类，表明免疫系统在改变龋齿微生物群中的作用。应怀疑PID组中突出的细菌种类和关联与当前或未来疾病的联系。
■参加本研究的是15名患有龋齿的原发性PID儿童（PID组）和15名患有龋齿的健康儿童作为对照（CG）。DMFT指数，唾液流速,在进行宏基因组分析之前,对每位参与者的缓冲能力进行了评估.对于分类分析，通过16SrRNA的V3-V4高变区的高通量测序获得读数.
■DMFT得分，唾液流速,各组的缓冲能力相似。流速和缓冲能力与物种数量无关，置信度为95％。宏基因组分析导致在所有样品中鉴定出2440种细菌。在相对丰度≥1%的50种最普遍的物种中，PID组中黑色素prevotella和唾液prevotella的含量不同。PID组和CG组显示出相似的物种丰富度和均匀度，但是Shannon-Weiner和InverseSimpson指数最高的5个样本中有4个属于PID组。PID亚组中物种相关性的Spearman检验结果表明，Prevotellaoris与Scardoviawiggziae和accertaesacgrances属均呈正相关。
■这项研究提供了对患有免疫缺陷疾病的儿童龋齿微生物群的见解。差异丰富的物种，新的细菌协会，在PID样本中发现了独特的细菌种类，表明免疫系统在改变龋齿微生物群中的作用。应怀疑PID组中突出的细菌种类和关联与当前或未来疾病的联系。
