PDF(Pubmed)
Abstract：
UNASSIGNED: The global obesity epidemic is a significant public health issue, often leading to metabolic disorders such as diabetes and cardiovascular diseases. Collagen peptides (CP) and their bioactive component, Prolyl-hydroxyproline (Pro-Hyp), have shown potential in reducing adipocyte size, with unclear mechanisms concerning brown adipocyte differentiation.
UNASSIGNED: We investigated the effects of Pro-Hyp on the differentiation of brown adipocytes in C3H10T1/2 mesenchymal stem cells, focusing on its impact on adipocyte size, gene expression related to brown fat function, and mitochondrial activity.
UNASSIGNED: Pro-Hyp treatment decreased adipocyte size and upregulated brown fat-specific genes, including C/EBPα, PGC-1α, and UCP-1. Remarkably, it did not alter PPARγ expression. Pro-Hyp also elevated mitochondrial activity, suggesting enhanced brown adipocyte functionality. A Pro-Hyp responsive element was identified in the PGC-1α gene promoter, which facilitated the binding of the Foxg1 transcription factor, indicating a novel regulatory mechanism.
UNASSIGNED: Pro-Hyp promotes brown adipocyte differentiation, potentially offering a therapeutic strategy for obesity management. This study provides a molecular basis for the anti-obesity effects of CP, although further in vivo studies are needed to confirm these findings and to investigate the potential impact on beige adipocyte differentiation.
UNASSIGNED: We investigated the effects of Pro-Hyp on the differentiation of brown adipocytes in C3H10T1/2 mesenchymal stem cells, focusing on its impact on adipocyte size, gene expression related to brown fat function, and mitochondrial activity.
UNASSIGNED: Pro-Hyp treatment decreased adipocyte size and upregulated brown fat-specific genes, including C/EBPα, PGC-1α, and UCP-1. Remarkably, it did not alter PPARγ expression. Pro-Hyp also elevated mitochondrial activity, suggesting enhanced brown adipocyte functionality. A Pro-Hyp responsive element was identified in the PGC-1α gene promoter, which facilitated the binding of the Foxg1 transcription factor, indicating a novel regulatory mechanism.
UNASSIGNED: Pro-Hyp promotes brown adipocyte differentiation, potentially offering a therapeutic strategy for obesity management. This study provides a molecular basis for the anti-obesity effects of CP, although further in vivo studies are needed to confirm these findings and to investigate the potential impact on beige adipocyte differentiation.
摘要：
■全球肥胖流行是一个重大的公共卫生问题，常导致代谢紊乱，如糖尿病和心血管疾病。胶原肽(CP)及其生物活性成分,脯氨酸-羟基脯氨酸（Pro-Hyp），已经显示出减少脂肪细胞大小的潜力，关于棕色脂肪细胞分化的机制不清楚。
■我们研究了Pro-Hyp对C3H10T1/2间充质干细胞中棕色脂肪细胞分化的影响，关注它对脂肪细胞大小的影响，与棕色脂肪功能相关的基因表达，和线粒体活动。
■Pro-Hyp治疗减少了脂肪细胞大小，上调了棕色脂肪特异性基因，包括C/EBPα，PGC-1α,UCP-1值得注意的是,它没有改变PPARγ的表达。Pro-Hyp也提高了线粒体活性，提示棕色脂肪细胞功能增强。在PGC-1α基因启动子中鉴定出一个Pro-Hyp反应元件，这促进了Foxg1转录因子的结合，表明了一种新的调控机制。
■Pro-Hyp促进棕色脂肪细胞分化，可能为肥胖管理提供治疗策略。本研究为CP的抗肥胖作用提供了分子基础,尽管需要进一步的体内研究来证实这些发现，并研究对米色脂肪细胞分化的潜在影响。
■我们研究了Pro-Hyp对C3H10T1/2间充质干细胞中棕色脂肪细胞分化的影响，关注它对脂肪细胞大小的影响，与棕色脂肪功能相关的基因表达，和线粒体活动。
■Pro-Hyp治疗减少了脂肪细胞大小，上调了棕色脂肪特异性基因，包括C/EBPα，PGC-1α,UCP-1值得注意的是,它没有改变PPARγ的表达。Pro-Hyp也提高了线粒体活性，提示棕色脂肪细胞功能增强。在PGC-1α基因启动子中鉴定出一个Pro-Hyp反应元件，这促进了Foxg1转录因子的结合，表明了一种新的调控机制。
■Pro-Hyp促进棕色脂肪细胞分化，可能为肥胖管理提供治疗策略。本研究为CP的抗肥胖作用提供了分子基础,尽管需要进一步的体内研究来证实这些发现，并研究对米色脂肪细胞分化的潜在影响。
