UNASSIGNED: We investigated the effects of Pro-Hyp on the differentiation of brown adipocytes in C3H10T1/2 mesenchymal stem cells, focusing on its impact on adipocyte size, gene expression related to brown fat function, and mitochondrial activity.
UNASSIGNED: Pro-Hyp treatment decreased adipocyte size and upregulated brown fat-specific genes, including C/EBPα, PGC-1α, and UCP-1. Remarkably, it did not alter PPARγ expression. Pro-Hyp also elevated mitochondrial activity, suggesting enhanced brown adipocyte functionality. A Pro-Hyp responsive element was identified in the PGC-1α gene promoter, which facilitated the binding of the Foxg1 transcription factor, indicating a novel regulatory mechanism.
UNASSIGNED: Pro-Hyp promotes brown adipocyte differentiation, potentially offering a therapeutic strategy for obesity management. This study provides a molecular basis for the anti-obesity effects of CP, although further in vivo studies are needed to confirm these findings and to investigate the potential impact on beige adipocyte differentiation.
■我们研究了Pro-Hyp对C3H10T1/2间充质干细胞中棕色脂肪细胞分化的影响,关注它对脂肪细胞大小的影响,与棕色脂肪功能相关的基因表达,和线粒体活动。
■Pro-Hyp治疗减少了脂肪细胞大小,上调了棕色脂肪特异性基因,包括C/EBPα,PGC-1α,UCP-1值得注意的是,它没有改变PPARγ的表达。Pro-Hyp也提高了线粒体活性,提示棕色脂肪细胞功能增强。在PGC-1α基因启动子中鉴定出一个Pro-Hyp反应元件,这促进了Foxg1转录因子的结合,表明了一种新的调控机制。
■Pro-Hyp促进棕色脂肪细胞分化,可能为肥胖管理提供治疗策略。本研究为CP的抗肥胖作用提供了分子基础,尽管需要进一步的体内研究来证实这些发现,并研究对米色脂肪细胞分化的潜在影响。