PDF(Pubmed)
Abstract：
UNASSIGNED: Recently, a flow cytometric (FC) based test has been developed for detection of circulating fetal cells to replace the less accurate and reproducible Kleihauer-Betke test.FC test is easier to perform, it can distinguish the origin of fetal cells, but it is expensive and available in highly specialized laboratories. We evaluated the introduction of high-performance liquid chromatography (HPLC) approach as initial screening to identify patients who need an additional FC test to better discriminate the nature of haemoglobin-F (HbF) positive cells.
UNASSIGNED: Blood samples from 130 pregnant women suspected to have fetomaternal haemorrhage were analysed with HPLC and FC methods. The cut-off for HbF HPLC concentration was calculated. Statistical analyses for the evaluation of HPLC as a screening method were performed. The positivity cut-off of HbF to be used as decision-making value to continue the investigation was calculated.
UNASSIGNED: An excellent agreement (R2 > 0.90) was observed between the percentage of HbF obtained by HPLC and the percentage of fetal cells detected by FC. Results obtained from each assay were compared to define the HPLC threshold below which it is not necessary to continue the investigations, confirming the maternal nature of the HbF positive cells detected. Our study demonstrated that a cut-off of 1.0 % HbF obtained by HPLC was associated with the lowest rate of false negative results in our patient cohort.
UNASSIGNED: This study provides a new FMH investigation approach that possibly leads to a reduction in times and costs of the analysis.
UNASSIGNED: Blood samples from 130 pregnant women suspected to have fetomaternal haemorrhage were analysed with HPLC and FC methods. The cut-off for HbF HPLC concentration was calculated. Statistical analyses for the evaluation of HPLC as a screening method were performed. The positivity cut-off of HbF to be used as decision-making value to continue the investigation was calculated.
UNASSIGNED: An excellent agreement (R2 > 0.90) was observed between the percentage of HbF obtained by HPLC and the percentage of fetal cells detected by FC. Results obtained from each assay were compared to define the HPLC threshold below which it is not necessary to continue the investigations, confirming the maternal nature of the HbF positive cells detected. Our study demonstrated that a cut-off of 1.0 % HbF obtained by HPLC was associated with the lowest rate of false negative results in our patient cohort.
UNASSIGNED: This study provides a new FMH investigation approach that possibly leads to a reduction in times and costs of the analysis.
摘要：
■最近，已经开发了一种基于流式细胞仪(FC)的检测方法,用于检测循环胎儿细胞,以取代不太准确和可重复的Kleihauer-Betke检测方法.FC测试更容易执行，它可以区分胎儿细胞的起源，但它很昂贵，可以在高度专业化的实验室获得。我们评估了引入高效液相色谱（HPLC）方法作为初步筛选的方法，以鉴定需要额外FC测试以更好地区分血红蛋白F（HbF）阳性细胞的性质的患者。
■用HPLC和FC方法分析了130名怀疑有母胎出血的孕妇的血样。计算HbFHPLC浓度的截止值。进行作为筛选方法的HPLC评价的统计分析。计算了用作继续调查的决策值的HbF的阳性截止值。
■在通过HPLC获得的HbF百分比与通过FC检测的胎儿细胞百分比之间观察到极好的一致性（R2>0.90）。将从每个测定中获得的结果进行比较，以定义HPLC阈值，低于该阈值无需继续研究。确认检测到的HbF阳性细胞的母体性质。我们的研究表明，在我们的患者队列中，通过HPLC获得的1.0%HbF的截止值与最低的假阴性结果率相关。
■这项研究提供了一种新的FMH调查方法，可能导致分析时间和成本的减少。
■用HPLC和FC方法分析了130名怀疑有母胎出血的孕妇的血样。计算HbFHPLC浓度的截止值。进行作为筛选方法的HPLC评价的统计分析。计算了用作继续调查的决策值的HbF的阳性截止值。
■在通过HPLC获得的HbF百分比与通过FC检测的胎儿细胞百分比之间观察到极好的一致性（R2>0.90）。将从每个测定中获得的结果进行比较，以定义HPLC阈值，低于该阈值无需继续研究。确认检测到的HbF阳性细胞的母体性质。我们的研究表明，在我们的患者队列中，通过HPLC获得的1.0%HbF的截止值与最低的假阴性结果率相关。
■这项研究提供了一种新的FMH调查方法，可能导致分析时间和成本的减少。
